K1421
Thermo Scientific™ GeneBLAzer™ ERR Alpha DA Assay Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1421-Each-of-1 | In Stock | ₹ 1,29,366.72 |
K1421 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,29,366.72
GST (18%): ₹ 23,286.01
Total Price: ₹ 1,52,652.73
Alternate Agonists
L165, 041, GW 1929, GW 7647, GW 0742
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ERR alpha HEK 293 T DA cells (K1421A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Gene ID (Entrez)
2101
Primary Agonist
Rosiglitazone
Quantity
1 plate
Target Entry
ESRRA, NR3B1, ERR1, ERR alpha
System Type
GeneBLAzer™
Antagonists
T0070907, GW 9662, GW 6471
Cell State
Division-Arrested Cells
Detection Method
Fluorescence
Gene Symbol
ERRα
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Cell Line
293T (HEK)
Druggable Target
Nuclear Receptors
Related Products
Description
- GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS)
- When an agonist binds to the LBD of the GAL4 (DBD)-ERR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase
- Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
- Both ERR alpha DA cells and ERR alpha-UAS-bla HEK 293T cells are functionally validated for Z' and EC 50 concentrations of XCT790, an inverse agonist
- In addition, ERR alpha-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
- Additional testing data using alternate stimuli are also available.
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Alternate Agonists: L165, 041, GW 1929, GW 7647, GW 0742
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ERR alpha HEK 293 T DA cells (K1421A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Gene ID (Entrez): 2101
Primary Agonist: Rosiglitazone
Quantity: 1 plate
Target Entry: ESRRA, NR3B1, ERR1, ERR alpha
System Type: GeneBLAzer™
Antagonists: T0070907, GW 9662, GW 6471
Cell State: Division-Arrested Cells
Detection Method: Fluorescence
Gene Symbol: ERRα
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: 293T (HEK)
Druggable Target: Nuclear Receptors
Alternate Agonists:
L165, 041, GW 1929, GW 7647, GW 0742
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ERR alpha HEK 293 T DA cells (K1421A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Gene ID (Entrez):
2101
Primary Agonist:
Rosiglitazone
Quantity:
1 plate
Target Entry:
ESRRA, NR3B1, ERR1, ERR alpha
System Type:
GeneBLAzer™
Antagonists:
T0070907, GW 9662, GW 6471
Cell State:
Division-Arrested Cells
Detection Method:
Fluorescence
Gene Symbol:
ERRα
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
293T (HEK)
Druggable Target:
Nuclear Receptors
Alternate Agonists: __
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells• GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Gene ID (Entrez): __
Primary Agonist: __
Quantity: 8 x 106Cells
Target Entry: CALCRL:RAMP3
System Type: GeneBLAzer™
Antagonists: __
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: CALCRL:RAMP3
Product Line: Freestyle™, GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: FreeStyle 293-F Cells
Druggable Target: GPCR
Alternate Agonists:
__
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells• GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Gene ID (Entrez):
__
Primary Agonist:
__
Quantity:
8 x 106Cells
Target Entry:
CALCRL:RAMP3
System Type:
GeneBLAzer™
Antagonists:
__
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
CALCRL:RAMP3
Product Line:
Freestyle™, GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
FreeStyle 293-F Cells
Druggable Target:
GPCR
Alternate Agonists: __
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ AGTR1-UAS-bla CHO-K1 cells• Tango™ AGTR1-UAS-bla CHO-K1 Assay Protocol• Certificate of AnalysisThe Tango™ AGTR1-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Gene ID (Entrez): __
Primary Agonist: __
Quantity: 8 x 106Cells
Target Entry: AGTR1
System Type: Tango™
Antagonists: __
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: AGTR1
Product Line: Tango™
Reporter Gene: __
Cell Line: U2OS
Druggable Target: GPCR
Alternate Agonists:
__
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ AGTR1-UAS-bla CHO-K1 cells• Tango™ AGTR1-UAS-bla CHO-K1 Assay Protocol• Certificate of AnalysisThe Tango™ AGTR1-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Gene ID (Entrez):
__
Primary Agonist:
__
Quantity:
8 x 106Cells
Target Entry:
AGTR1
System Type:
Tango™
Antagonists:
__
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
AGTR1
Product Line:
Tango™
Reporter Gene:
__
Cell Line:
U2OS
Druggable Target:
GPCR
Alternate Agonists: __
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ EDNRA-bla U2OS cells• Tango™ EDNRA-bla U2OS Assay Protocol• Certificate of AnalysisTango™ EDNRA-bla U2OS cells are shipped on dry ice. Transfer to liquid nitrogen immediately upon receipt or thaw for immediate use.
Gene ID (Entrez): __
Primary Agonist: __
Quantity: 2 x 106Cells
Target Entry: EDNRA
System Type: Tango™
Antagonists: __
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: EDNRA
Product Line: Tango™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: U2OS
Druggable Target: GPCR
Alternate Agonists:
__
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ EDNRA-bla U2OS cells• Tango™ EDNRA-bla U2OS Assay Protocol• Certificate of AnalysisTango™ EDNRA-bla U2OS cells are shipped on dry ice. Transfer to liquid nitrogen immediately upon receipt or thaw for immediate use.
Gene ID (Entrez):
__
Primary Agonist:
__
Quantity:
2 x 106Cells
Target Entry:
EDNRA
System Type:
Tango™
Antagonists:
__
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
EDNRA
Product Line:
Tango™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
U2OS
Druggable Target:
GPCR