K1407
Thermo Scientific™ GeneBLAzer™ RAR-beta DA Assay Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1407-Each-of-1 | In Stock | ₹ 1,31,542.00 |
K1407 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,31,542.00
GST (18%): ₹ 23,677.56
Total Price: ₹ 1,55,219.56
Alternate Agonists
Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:• RAR beta HEK 293T DA cells (K1407A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene Symbol
RARβ
Quantity
1 plate
Target Entry
RAR beta, NR1B2, RARB
Cell Line
293T (HEK)
Druggable Target
Nuclear Receptors
Antagonists
none
Cell State
Division-Arrested Cells
Detection Method
Fluorescence
Primary Agonist
9-cis-retinoic acid
Reporter Gene
BLA (Beta-Lactamase)
Type
Division-Arrested Cells Assay Kit
System Type
GeneBLAzer™
Related Products
Description
- GeneBLAzer™RAR beta DA (Division Arrested) cells and RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line
- GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS)
- When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase
- DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
- Both RAR beta DA cells and RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC 50 concentrations of all-trans retinoic acid (ATRA)
- In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
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Alternate Agonists: Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:• RAR beta HEK 293T DA cells (K1407A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene Symbol: RARβ
Quantity: 1 plate
Target Entry: RAR beta, NR1B2, RARB
Cell Line: 293T (HEK)
Druggable Target: Nuclear Receptors
Antagonists: none
Cell State: Division-Arrested Cells
Detection Method: Fluorescence
Primary Agonist: 9-cis-retinoic acid
Reporter Gene: BLA (Beta-Lactamase)
Type: Division-Arrested Cells Assay Kit
System Type: GeneBLAzer™
Alternate Agonists:
Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:• RAR beta HEK 293T DA cells (K1407A)• LiveBLAzer™-FRET B/G Loading Kit, 70 μgShipping:Dry IceStorage:-80°C (liquid nitrogen)
Gene Symbol:
RARβ
Quantity:
1 plate
Target Entry:
RAR beta, NR1B2, RARB
Cell Line:
293T (HEK)
Druggable Target:
Nuclear Receptors
Antagonists:
none
Cell State:
Division-Arrested Cells
Detection Method:
Fluorescence
Primary Agonist:
9-cis-retinoic acid
Reporter Gene:
BLA (Beta-Lactamase)
Type:
Division-Arrested Cells Assay Kit
System Type:
GeneBLAzer™
Alternate Agonists: L165, 041, GW 1929, GW 7647, GW 0742
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ERR alpha HEK 293 T DA cells (K1421A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Gene Symbol: ERRα
Quantity: 1 plate
Target Entry: ESRRA, NR3B1, ERR1, ERR alpha
Cell Line: 293T (HEK)
Druggable Target: Nuclear Receptors
Antagonists: T0070907, GW 9662, GW 6471
Cell State: Division-Arrested Cells
Detection Method: Fluorescence
Primary Agonist: Rosiglitazone
Reporter Gene: BLA (Beta-Lactamase)
Type: __
System Type: GeneBLAzer™
Alternate Agonists:
L165, 041, GW 1929, GW 7647, GW 0742
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ERR alpha HEK 293 T DA cells (K1421A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Gene Symbol:
ERRα
Quantity:
1 plate
Target Entry:
ESRRA, NR3B1, ERR1, ERR alpha
Cell Line:
293T (HEK)
Druggable Target:
Nuclear Receptors
Antagonists:
T0070907, GW 9662, GW 6471
Cell State:
Division-Arrested Cells
Detection Method:
Fluorescence
Primary Agonist:
Rosiglitazone
Reporter Gene:
BLA (Beta-Lactamase)
Type:
__
System Type:
GeneBLAzer™
Alternate Agonists: __
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells• GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Gene Symbol: CALCRL:RAMP3
Quantity: 8 x 106Cells
Target Entry: CALCRL:RAMP3
Cell Line: FreeStyle 293-F Cells
Druggable Target: GPCR
Antagonists: __
Cell State: Dividing Cells
Detection Method: Fluorescence
Primary Agonist: __
Reporter Gene: BLA (Beta-Lactamase)
Type: __
System Type: GeneBLAzer™
Alternate Agonists:
__
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells• GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Gene Symbol:
CALCRL:RAMP3
Quantity:
8 x 106Cells
Target Entry:
CALCRL:RAMP3
Cell Line:
FreeStyle 293-F Cells
Druggable Target:
GPCR
Antagonists:
__
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Primary Agonist:
__
Reporter Gene:
BLA (Beta-Lactamase)
Type:
__
System Type:
GeneBLAzer™
Alternate Agonists: __
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ AGTR1-UAS-bla CHO-K1 cells• Tango™ AGTR1-UAS-bla CHO-K1 Assay Protocol• Certificate of AnalysisThe Tango™ AGTR1-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Gene Symbol: AGTR1
Quantity: 8 x 106Cells
Target Entry: AGTR1
Cell Line: U2OS
Druggable Target: GPCR
Antagonists: __
Cell State: Dividing Cells
Detection Method: Fluorescence
Primary Agonist: __
Reporter Gene: __
Type: __
System Type: Tango™
Alternate Agonists:
__
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ AGTR1-UAS-bla CHO-K1 cells• Tango™ AGTR1-UAS-bla CHO-K1 Assay Protocol• Certificate of AnalysisThe Tango™ AGTR1-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Gene Symbol:
AGTR1
Quantity:
8 x 106Cells
Target Entry:
AGTR1
Cell Line:
U2OS
Druggable Target:
GPCR
Antagonists:
__
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Primary Agonist:
__
Reporter Gene:
__
Type:
__
System Type:
Tango™