Thermo Scientific™ GeneBLAzer™ CRHR1 CHO-K1 DA Assay Kit

Description

• Division Arrested (DA) cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction
• Both CRHR1 CHO-K1 DA cells and CRHR1-CRE- bla CHO-K1 cells are functionally validated for Z’-factor and EC 50 concentrations of corticotropin releasing factor (CRF), (Figure 1)
• In addition, CRHR1-CRE- bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request)
• Additional testing data using alternate stimuli are also available
• Corticotropin releasing factor is a 41-amino acid peptide that plays a role in the integration of autonomic, neuroendocrine, and behavioral responses to stress
• These effects are mediated through two receptor families, CRHR1 and CRHR2
• While CRF was originally isolated from the hypothalamus, where it was shown to be the primary neuroregulator mediating the hypothalamic-pituitary-adrenocortical stress axis, it has since been found to be widely distributed outside the hypothalamus throughout the central nervous system
• Presently, there are five distinct targets for CRF with unique pharmacology and localization
• These have been placed into three distinct classes, two of which are the G-protein-coupled receptors CRF1 (CRHR1) and CRF2 (CRHR2)
• Three functional splice variants have been identified for the mammalian CRHR2 receptor, although pharmacological characterization of these splice variants has revealed no major differences between them
• GeneBLAzer™ CRHR1 CHO-K1 DA Cells and CRHR1-CRE- bla CHO-K1 Cells are shipped on dry ice
• Store in liquid nitrogen immediately upon receipt or thaw for immediate use
• Contents: GeneBLAzer™ CRHR1 CHO-K1 DA Assay Kit Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate
• (Other materials are required separately; please refer to the protocol)
• Includes: •CRHR1 CHO-K1 DA cells •LiveBLAzer™-FRET B/G Loading Kit, 70μg •Solution D, 1ml •2 protocols •Certificate of Analysis GeneBLAzer™ CRHR1 CHO-K1 DA cells Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol)
• Includes: •CRHR1 CHO-K1 DA cells •1 protocol •Certificate of Analysis GeneBLAzer™ CRHR1-CRE- bla CHO-K1 cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol)
• Includes: •CRHR1-CRE- bla CHO-K1 cells •1 protocol •Certificate of Analysis