Thermo Scientific™ LanthaScreen™ ATF2 (19-106) A549 Cell Line

Description

• The LanthaScreen™ATF2 (19-106) A549 cell line contains a fusion protein consisting of GFP and a fragment encoding for aa 19-106 of ATF2 under the control of a CMV promotor
• A549 is a lung carcinoma cell line which shows inducible activation of JNK by number of different ligands, such as TNF and EGF, which leads to the transient phosphorylation of GFP-ATF2 (19-106)
• This LanthaScreen™cell line therefore allows the analysis of JNK activity using GFP-ATF2 phosphorylation as readout
• The GFP-ATF2 (19-106) construct was transfected into A549 cells using Lipofectamine™2000, followed by selection with Blasticidin
• This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as sorting marker
• This cell line is validated for EC50 and Z' under optimized conditions using TNF as ligand for JNK mediated GFP-ATF2 (19-106) phosphorylation
• This cell line has been tested for assay performance under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance and assay development time.