Thermo Scientific™ LanthaScreen™ IkB alpha GripTite™ Cell Line

Description

• The LanthaScreen™ IkB alpha GripTite™ cell line contains a fusion protein consisting of the cDNA encoding for GFP and IkB (GFP is fused to the N-terminus of IkB alpha) under the control of a CMV promoter stably transfected into GripTite™ cells
• GripTite™ is a modified HEK293 cell line, which is a human kidney cell line which shows inducible activation of the NFkB signaling pathway by a number of different ligands, such as TNF and IL-1
• Activation of the NFkB pathway involves the activation of IKK resulting in the phosphorylation of its target protein IkB alpha
• This LanthaScreen cell line allows therefore the analysis of IKK activity using GFP-IkB alpha phosphorylation as readout
• The GFP-IkB alpha construct was transfected into GripTite™ cells using Lipofectamine™ 2000, followed by selection with Blasticidin
• This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as sorting marker
• This cell line is validated for EC50 and Z' under optimized conditions using TNF as ligand for IKK mediated GFP-IkB alpha phosphorylation or ubiquitination
• This cell line has also been tested for assay performance under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance and assay development time
• Additional information using alternate stimuli is also available
• Academic and non-profit customers, please inquire for special pricing.