K1737
Thermo Scientific™ GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 Cells
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1737-Each-of-1 | In Stock | ₹ 8,88,112.80 |
K1737 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 8,88,112.80
GST (18%): ₹ 1,59,860.304
Total Price: ₹ 10,47,973.104
Accession Number
NM_001526.2
Cell State
Dividing Cells
Detection Method
Fluorescence
Gene Symbol
HCRTR2
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Cell Line
CHO-K1
Druggable Target
GPCR
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 cells• GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application)
FRET
Population Status
Single Cell Clone Origin
Quantity
2 x 106Cells
Target Entry
HCRTR2
System Type
GeneBLAzer™
Related Products
Description
- GeneBLAzer™ HCRTR2-Gα15-NFAT- bla CHO-K1 cells contain the human Orexin Receptor B (HCRTR2) stably integrated into the GeneBLAzer™ Gα15-NFAT- bla CHO-K1 cell line
- GeneBLAzer™ Gα15-NFAT- bla CHO-K1 cells (Cat
- No
- K1537) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15
- The GeneBLAzer™ HCRTR2-Gα15-NFAT- bla CHO-K1 cells are functionally validated for Z' and EC 50 concentrations of Orexin B
- In addition, GeneBLAzer™ HCRTR2-Gα15-NFAT- bla CHO-K1 cells have been tested for assay performance under variable conditions
- These data are found in the Validation & Assay Performance Summary
- The HCRTR2 receptor has been detected in the brain as well as adrenal gland, gastrointestinal tract, and pancreas
- The signaling cascades used by orexin receptors appear to be varied and cell type dependant
- In CHO cells, calcium release is seen after agonist treatment
- In hypothalamic neurons, the frequency of action potentials is increased
- Administering either orexin peptide into the bloodstream of rats caused an increase in feeding behavior
- The orexin system is also thought to regulate neuroendocrine function and the sleep wake cycle
- HCRTR2 has been directly implicated in narcolepsy in animal studies
- For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
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Accession Number: NM_001526.2
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: HCRTR2
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Druggable Target: GPCR
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 cells• GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Population Status: Single Cell Clone Origin
Quantity: 2 x 106Cells
Target Entry: HCRTR2
System Type: GeneBLAzer™
Accession Number:
NM_001526.2
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
HCRTR2
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
CHO-K1
Druggable Target:
GPCR
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 cells• GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ HCRTR2-Gα15-NFAT-blaCHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Population Status:
Single Cell Clone Origin
Quantity:
2 x 106Cells
Target Entry:
HCRTR2
System Type:
GeneBLAzer™
Accession Number: NM_000953.2
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: PTGDR
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Druggable Target: GPCR
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: Contents:K1739 GeneBLAzer™ PTGDR-CRE-blaCHO-K1 cells(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).Includes:• GeneBLAzer™ PTGDR-CRE-blaCHO-K1 cells• GeneBLAzer™ PTGDR-CRE-blaCHO-K1 Assay Protocol• Certificate of AnalysisStorage:The GeneBLAzer™ PTGDR-CRE-blaCHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Population Status: Single Cell Clone Origin
Quantity: 2 x 106Cells
Target Entry: PTGDR
System Type: GeneBLAzer™
Accession Number:
NM_000953.2
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
PTGDR
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
CHO-K1
Druggable Target:
GPCR
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
Contents:K1739 GeneBLAzer™ PTGDR-CRE-blaCHO-K1 cells(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).Includes:• GeneBLAzer™ PTGDR-CRE-blaCHO-K1 cells• GeneBLAzer™ PTGDR-CRE-blaCHO-K1 Assay Protocol• Certificate of AnalysisStorage:The GeneBLAzer™ PTGDR-CRE-blaCHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Population Status:
Single Cell Clone Origin
Quantity:
2 x 106Cells
Target Entry:
PTGDR
System Type:
GeneBLAzer™
Accession Number: __
Cell State: __
Detection Method: __
Gene Symbol: __
Product Line: EasySelect™
Reporter Gene: __
Cell Line: __
Druggable Target: __
Assay Entry: __
Content And Storage: The EasySelectPichiaExpression Kit includes 20 μg each of the plasmids pPICZ A, B, and C and pPICZα A, B, and C;Pichia pastorisstrains X-33 (wild-type), GS115 (his4), KM71H (arg4 aox1::ARG4), and control strains for both intracellular and secreted expression; 250 mg Zeocin™ antibiotic; media (YP and YNB), EasyComp™PichiaTransformation Kit, 2 μg each 5'AOX1, 3'AOX1, and α-factor sequencing primers. The pPICZ and pPICZα vectors are also available separately. AlacZ positive expression control is provided with pPICZ.Store strains at +4°C. Store vectors and primers at -20°C. All components are guaranteed stable for 6 months when properly stored.
For Use With (Application): Protein Expression
Population Status: __
Quantity: 1 Kit
Target Entry: __
System Type: __
Accession Number:
__
Cell State:
__
Detection Method:
__
Gene Symbol:
__
Product Line:
EasySelect™
Reporter Gene:
__
Cell Line:
__
Druggable Target:
__
Assay Entry:
__
Content And Storage:
The EasySelectPichiaExpression Kit includes 20 μg each of the plasmids pPICZ A, B, and C and pPICZα A, B, and C;Pichia pastorisstrains X-33 (wild-type), GS115 (his4), KM71H (arg4 aox1::ARG4), and control strains for both intracellular and secreted expression; 250 mg Zeocin™ antibiotic; media (YP and YNB), EasyComp™PichiaTransformation Kit, 2 μg each 5'AOX1, 3'AOX1, and α-factor sequencing primers. The pPICZ and pPICZα vectors are also available separately. AlacZ positive expression control is provided with pPICZ.Store strains at +4°C. Store vectors and primers at -20°C. All components are guaranteed stable for 6 months when properly stored.
For Use With (Application):
Protein Expression
Population Status:
__
Quantity:
1 Kit
Target Entry:
__
System Type:
__
Accession Number: NM_000741
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: M4
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Druggable Target: GPCR
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ M4 Gqo5 CHO-K1 DA cells• GeneBLAzer™ M4 Gqo5 CHO-K1 DA Assay Protocol• Certificate of Analysis• Solution D• LiveBLAzer™-FRET B/G Loading ProtocolThe GeneBLAzer™ M4-Gqo5-NFAT-blaCHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Population Status: Single Cell Clone Origin
Quantity: 2 x 106Cells
Target Entry: M4
System Type: GeneBLAzer™
Accession Number:
NM_000741
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
M4
Product Line:
GeneBLAzer™
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
CHO-K1
Druggable Target:
GPCR
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ M4 Gqo5 CHO-K1 DA cells• GeneBLAzer™ M4 Gqo5 CHO-K1 DA Assay Protocol• Certificate of Analysis• Solution D• LiveBLAzer™-FRET B/G Loading ProtocolThe GeneBLAzer™ M4-Gqo5-NFAT-blaCHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Population Status:
Single Cell Clone Origin
Quantity:
2 x 106Cells
Target Entry:
M4
System Type:
GeneBLAzer™