K1813
Thermo Scientific™ CellSensor™ HSE-blaHeLa Cell Line
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1813-Each-of-1 | In Stock | ₹ 12,81,688.80 |
K1813 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 12,81,688.80
GST (18%): ₹ 2,30,703.984
Total Price: ₹ 15,12,392.784
Antagonists
Quercetin
Cell State
Dividing Cells
Detection Method
Fluorescence
Gene Symbol
HSE
Product Line
CellSensor™
Readout
End Point
Target Entry
Heat shock response pathway, HSF-1
System Type
CellSensor™
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
CellSensor™ HSE-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application)
FRET
Primary Agonist
17-AAG
Quantity
1 Vial
Reporter Gene
BLA (Beta-Lactamase)
Cell Line
HeLa
Druggable Target
Signaling Pathway
Related Products
Description
- Activation of the heat shock response/unfolded protein response (HSR/UPR) occurs in response to a diversity of chemical, environmental, and physiological stress conditions
- Transcriptional regulation of the human HSR is mediated by a family of three heat shock transcription factors (HSFs), HSF-1, -2, and -4
- Stress-induced activation of quiescent HSF monomers results in their trimerization and accumulation in the nucleus, wherein they bind to and upregulate transcription of target genes (e.g
- molecular chaperones, certain proteases, and other stress response genes) harboring a heat shock element (HSE)
- Downstream expression of heat shock protein family members (e.g
- Hsp90 and Hsp70) that function as molecular chaperones to guide conformational states of client proteins is essential to maintaining the health of cells and protecting them from various acute and chronic stress conditions
- As a result, HSR activation may provide therapeutic benefit to certain types of tissue trauma (e.g
- brain and heart ischemia) and neurodegenerative disorders (e.g
- Huntington disease, Alzheimer disease, and Parkinson disease)
- Conversely, since aberrant expression of chaperones has been associated with tumorigenesis, compounds that down-regulate the HSR and chaperone levels could provide useful tools for combating cancer
- To generate an effective readout for interrogating the HSR pathway, we engineered HeLa cervical cancer cells with an HSE driving beta-lactamase reporter gene expression (HSE-bla)
- A stably integrated pool of heat shock responsive cells was isolated by FACS and further evaluated using an inhibitor of Hsp90, 17-AAG
- Hsp90 inhibition is known to upregulate HSF-1 activity leading to potent induction of the HSR, which can be readout using HSE-bla HeLa cells.
Compare Similar Items
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Antagonists: Quercetin
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: HSE
Product Line: CellSensor™
Readout: End Point
Target Entry: Heat shock response pathway, HSF-1
System Type: CellSensor™
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: CellSensor™ HSE-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application): FRET
Primary Agonist: 17-AAG
Quantity: 1 Vial
Reporter Gene: BLA (Beta-Lactamase)
Cell Line: HeLa
Druggable Target: Signaling Pathway
Antagonists:
Quercetin
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
HSE
Product Line:
CellSensor™
Readout:
End Point
Target Entry:
Heat shock response pathway, HSF-1
System Type:
CellSensor™
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
CellSensor™ HSE-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application):
FRET
Primary Agonist:
17-AAG
Quantity:
1 Vial
Reporter Gene:
BLA (Beta-Lactamase)
Cell Line:
HeLa
Druggable Target:
Signaling Pathway
Antagonists: __
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: CCR3
Product Line: GeneBLAzer™, Tango™
Readout: End Point
Target Entry: CCR3
System Type: Tango™
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ CCR3-bla U2OS cells• Tango™ CCR3-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ CCR3-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Primary Agonist: __
Quantity: 8 x 106Cells
Reporter Gene: __
Cell Line: U2OS
Druggable Target: GPCR
Antagonists:
__
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
CCR3
Product Line:
GeneBLAzer™, Tango™
Readout:
End Point
Target Entry:
CCR3
System Type:
Tango™
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ CCR3-bla U2OS cells• Tango™ CCR3-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ CCR3-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Primary Agonist:
__
Quantity:
8 x 106Cells
Reporter Gene:
__
Cell Line:
U2OS
Druggable Target:
GPCR
Antagonists: Salubrinal
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: ESRE
Product Line: CellSensor™
Readout: End Point
Target Entry: ATF6, ER stress response pathway
System Type: CellSensor™
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: CellSensor™ ESRE-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application): FRET
Primary Agonist: Tunicamycin
Quantity: 1 Vial
Reporter Gene: BLA (Beta-Lactamase), BLA (Beta-lactamase)
Cell Line: HeLa
Druggable Target: Signaling Pathway
Antagonists:
Salubrinal
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
ESRE
Product Line:
CellSensor™
Readout:
End Point
Target Entry:
ATF6, ER stress response pathway
System Type:
CellSensor™
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
CellSensor™ ESRE-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application):
FRET
Primary Agonist:
Tunicamycin
Quantity:
1 Vial
Reporter Gene:
BLA (Beta-Lactamase), BLA (Beta-lactamase)
Cell Line:
HeLa
Druggable Target:
Signaling Pathway
Antagonists: __
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: M4
Product Line: GeneBLAzer™, Tango™
Readout: End Point
Target Entry: M4
System Type: Tango™
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ M4-bla U2OS cells• Tango™ M4-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ M4-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Primary Agonist: __
Quantity: 8 x 106Cells
Reporter Gene: __
Cell Line: U2OS
Druggable Target: GPCR
Antagonists:
__
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
M4
Product Line:
GeneBLAzer™, Tango™
Readout:
End Point
Target Entry:
M4
System Type:
Tango™
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ M4-bla U2OS cells• Tango™ M4-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ M4-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Primary Agonist:
__
Quantity:
8 x 106Cells
Reporter Gene:
__
Cell Line:
U2OS
Druggable Target:
GPCR