K1838
Thermo Scientific™ CellSensor™ Gli-bla22Rv.1 Cell Line
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1838-Each-of-1 | In Stock | ₹ 9,41,620.00 |
K1838 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,41,620.00
GST (18%): ₹ 1,69,491.60
Total Price: ₹ 11,11,111.60
Antagonists
KAAD-Cyclopamine
Cell State
Dividing Cells
Detection Method
Fluorescence
Gene Symbol
GLI
Primary Agonist
Constitutive
Quantity
1 vial
Reporter Gene
BLA (Beta-Lactamase), BLA (Beta-lactamase)
Type
Cell Line
System Type
CellSensor™
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
CellSensor™ Gli-bla 22Rv.1 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application)
FRET
Human Protein Class
Cell Signalling
Product Line
CellSensor™
Readout
End Point
Target Entry
SMO, Hedgehog pathway
Cell Line
22Rv.1
Druggable Target
Signaling Pathway
Related Products
Description
- The morphogenic signal Shh provides in the developing CNS induces proliferation of neuronal precursor cells in the developing cerebellum and other tissues
- Proliferative signaling by Shh is involved in the development of cancer, including specific brain and skin cancers such as basal cell carcinomas
- Signaling takes place through a Patched (PTC-1)/Smoothened (SMO) Receptor complex
- The activation of Patched by Shh releases the inhibition of Patched on Smoothened leading to Sonic Hedgehog pathway activation of the Gli transcription factor to induce downstream gene expression
- The CellSensor™ Gli-bla 22Rv1 cell line contains a beta-lactamase reporter gene under control of the Gli response element stably integrated into 22Rv1 cells
- This cell line is a clonal population isolated by flow cytometry
- The clone was selected based on inhibition of the pathway with KAAD-Cyclopamine
- This cell line has also been tested for assay performance under variable conditions, including DMSO concentration, cell number, compound incubation time, and substrate loading time and validated for Z' under maximal inhibition of β-lactamase activity with Clavulanic Acid.
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Antagonists: KAAD-Cyclopamine
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: GLI
Primary Agonist: Constitutive
Quantity: 1 vial
Reporter Gene: BLA (Beta-Lactamase), BLA (Beta-lactamase)
Type: Cell Line
System Type: CellSensor™
Assay Entry: Cell-based beta lactamase reporter gene
Content And Storage: CellSensor™ Gli-bla 22Rv.1 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application): FRET
Human Protein Class: Cell Signalling
Product Line: CellSensor™
Readout: End Point
Target Entry: SMO, Hedgehog pathway
Cell Line: 22Rv.1
Druggable Target: Signaling Pathway
Antagonists:
KAAD-Cyclopamine
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
GLI
Primary Agonist:
Constitutive
Quantity:
1 vial
Reporter Gene:
BLA (Beta-Lactamase), BLA (Beta-lactamase)
Type:
Cell Line
System Type:
CellSensor™
Assay Entry:
Cell-based beta lactamase reporter gene
Content And Storage:
CellSensor™ Gli-bla 22Rv.1 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application):
FRET
Human Protein Class:
Cell Signalling
Product Line:
CellSensor™
Readout:
End Point
Target Entry:
SMO, Hedgehog pathway
Cell Line:
22Rv.1
Druggable Target:
Signaling Pathway
Antagonists: __
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: GPR23
Primary Agonist: __
Quantity: 8 x 106Cells
Reporter Gene: __
Type: U2OS Cell
System Type: Tango™
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ GPR23-UAS-bla CHO-K1 cells• Tango™ GPR23-UAS-bla CHO-K1 Assay Protocol• Certificate of AnalysisThe Tango™ GPR23-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Product Line: Tango™
Readout: End Point
Target Entry: GPR23
Cell Line: U2OS
Druggable Target: GPCR
Antagonists:
__
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
GPR23
Primary Agonist:
__
Quantity:
8 x 106Cells
Reporter Gene:
__
Type:
U2OS Cell
System Type:
Tango™
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ GPR23-UAS-bla CHO-K1 cells• Tango™ GPR23-UAS-bla CHO-K1 Assay Protocol• Certificate of AnalysisThe Tango™ GPR23-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Human Protein Class:
G-Protein Coupled Receptor (GPCR)
Product Line:
Tango™
Readout:
End Point
Target Entry:
GPR23
Cell Line:
U2OS
Druggable Target:
GPCR
Antagonists: __
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: GPR39
Primary Agonist: __
Quantity: 8 x 106Cells
Reporter Gene: __
Type: __
System Type: Tango™
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ GPR39-UAS-bla CHO-K1 cells• Tango™ GPR39-UAS-bla CHO-K1 Assay Protocol• Certificate of AnalysisThe Tango™ GPR39-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Product Line: Tango™
Readout: End Point
Target Entry: GPR39
Cell Line: U2OS
Druggable Target: GPCR
Antagonists:
__
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
GPR39
Primary Agonist:
__
Quantity:
8 x 106Cells
Reporter Gene:
__
Type:
__
System Type:
Tango™
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ GPR39-UAS-bla CHO-K1 cells• Tango™ GPR39-UAS-bla CHO-K1 Assay Protocol• Certificate of AnalysisThe Tango™ GPR39-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Human Protein Class:
G-Protein Coupled Receptor (GPCR)
Product Line:
Tango™
Readout:
End Point
Target Entry:
GPR39
Cell Line:
U2OS
Druggable Target:
GPCR
Antagonists: __
Cell State: Dividing Cells
Detection Method: Fluorescence
Gene Symbol: GPR120S
Primary Agonist: __
Quantity: 8 x 106Cells
Reporter Gene: __
Type: U2OS Cell
System Type: Tango™
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D, and other materials are required separately; please refer to the protocol.Includes:• Tango™ GPR120S-bla U2OS cells• Tango™ GPR120S-bla U2OS cell Assay Protocol• Certificate of AnalysisThe Tango™ GPR120S-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application): FRET
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Product Line: Tango™
Readout: End Point
Target Entry: GPR120S
Cell Line: U2OS
Druggable Target: GPCR
Antagonists:
__
Cell State:
Dividing Cells
Detection Method:
Fluorescence
Gene Symbol:
GPR120S
Primary Agonist:
__
Quantity:
8 x 106Cells
Reporter Gene:
__
Type:
U2OS Cell
System Type:
Tango™
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D, and other materials are required separately; please refer to the protocol.Includes:• Tango™ GPR120S-bla U2OS cells• Tango™ GPR120S-bla U2OS cell Assay Protocol• Certificate of AnalysisThe Tango™ GPR120S-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application):
FRET
Human Protein Class:
G-Protein Coupled Receptor (GPCR)
Product Line:
Tango™
Readout:
End Point
Target Entry:
GPR120S
Cell Line:
U2OS
Druggable Target:
GPCR