K1818
Thermo Scientific™ Tango™ M4-blaU2OS Cells
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1818-Each-of-1 | In Stock | ₹ 9,05,224.80 |
K1818 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,05,224.80
GST (18%): ₹ 1,62,940.464
Total Price: ₹ 10,68,165.264
Accession Number
NM_000741
Cell State
Dividing Cells
Cytoplasmic Tail
Native Tail
For Use With (Application)
FRET
Human Protein Class
G-Protein Coupled Receptor (GPCR)
Product Line
GeneBLAzer™, Tango™
Readout
End Point
Type
U2OS Cell
System Type
Tango™
Assay Entry
Cell-based beta-arrestin recruitment
Content And Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ M4-bla U2OS cells• Tango™ M4-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ M4-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method
Fluorescence
Gene Symbol
M4
Population Status
Single Cell Clone Origin
Quantity
8 x 106Cells
Target Entry
M4
Cell Line
U2OS
Druggable Target
GPCR
Related Products
Description
- The Tango™ M4-bla U2OS cells contain the human Acetycholine (muscarinic) subtype 4 receptor (M4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line
- This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element
- The Tango™ M4-bla U2OS cells are functionally validated for Z' and EC50 concentrations of Carbachol
- In addition, Tango™ M4-bla U2OS cells have been tested for assay performance under variable conditions
- These data are found in the Validation & Assay Performance Summary
- For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
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Accession Number: NM_000741
Cell State: Dividing Cells
Cytoplasmic Tail: Native Tail
For Use With (Application): FRET
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Product Line: GeneBLAzer™, Tango™
Readout: End Point
Type: U2OS Cell
System Type: Tango™
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ M4-bla U2OS cells• Tango™ M4-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ M4-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method: Fluorescence
Gene Symbol: M4
Population Status: Single Cell Clone Origin
Quantity: 8 x 106Cells
Target Entry: M4
Cell Line: U2OS
Druggable Target: GPCR
Accession Number:
NM_000741
Cell State:
Dividing Cells
Cytoplasmic Tail:
Native Tail
For Use With (Application):
FRET
Human Protein Class:
G-Protein Coupled Receptor (GPCR)
Product Line:
GeneBLAzer™, Tango™
Readout:
End Point
Type:
U2OS Cell
System Type:
Tango™
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ M4-bla U2OS cells• Tango™ M4-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ M4-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method:
Fluorescence
Gene Symbol:
M4
Population Status:
Single Cell Clone Origin
Quantity:
8 x 106Cells
Target Entry:
M4
Cell Line:
U2OS
Druggable Target:
GPCR
Accession Number: __
Cell State: __
Cytoplasmic Tail: __
For Use With (Application): Genotyping, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing, PCR
Human Protein Class: __
Product Line: PureLink™
Readout: __
Type: __
System Type: __
Assay Entry: __
Content And Storage: • 2.0 mL PureLink™ Genomic Lysis/Binding Buffer• 1.8 mL PureLink™ Genomic Digestion Buffer• 2.0 mL PureLink™ Genomic Wash Buffer 1• 1.5 mL PureLink™ Genomic Wash Buffer 2• 2.0 mL PureLink™ Genomic Elution Buffer• 0.4 mL RNase A (20 mg/mL)• 0.2 mL Proteinase K (20 mg/mL)• 10 PureLink™ Spin Columns with Collection Tubes• 20 PureLink™ Collecion Tubes (2.0 mL)Store all components at room temperature.
Detection Method: __
Gene Symbol: __
Population Status: __
Quantity: 10 Preps
Target Entry: __
Cell Line: __
Druggable Target: __
Accession Number:
__
Cell State:
__
Cytoplasmic Tail:
__
For Use With (Application):
Genotyping, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing, PCR
Human Protein Class:
__
Product Line:
PureLink™
Readout:
__
Type:
__
System Type:
__
Assay Entry:
__
Content And Storage:
• 2.0 mL PureLink™ Genomic Lysis/Binding Buffer• 1.8 mL PureLink™ Genomic Digestion Buffer• 2.0 mL PureLink™ Genomic Wash Buffer 1• 1.5 mL PureLink™ Genomic Wash Buffer 2• 2.0 mL PureLink™ Genomic Elution Buffer• 0.4 mL RNase A (20 mg/mL)• 0.2 mL Proteinase K (20 mg/mL)• 10 PureLink™ Spin Columns with Collection Tubes• 20 PureLink™ Collecion Tubes (2.0 mL)Store all components at room temperature.
Detection Method:
__
Gene Symbol:
__
Population Status:
__
Quantity:
10 Preps
Target Entry:
__
Cell Line:
__
Druggable Target:
__
Accession Number: __
Cell State: __
Cytoplasmic Tail: __
For Use With (Application): Genotyping, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing, PCR
Human Protein Class: __
Product Line: PureLink™
Readout: __
Type: __
System Type: __
Assay Entry: __
Content And Storage: • 10 mL PureLink™ Genomic Lysis/Binding Buffer• 9 mL PureLink™ Genomic Digestion Buffer• 10 mL PureLink™ Genomic Wash Buffer 1• 7.5 mL PureLink™ Genomic Wash Buffer 2• 10 mL PureLink™ Genomic Elution Buffer• 1 mL RNase A (20 mg/mL)• 1 mL Proteinase K (20 mg/mL)• 50 each PureLink™ Spin Columns with Collection Tubes• 100 PureLink™ Collecion Tubes (2.0 mL)This kit contains enough reagents for 50 DNA preps. Store all components at room temperature. For long-term storage, Proteinase K and RNase A may be stored at 4°C.
Detection Method: __
Gene Symbol: __
Population Status: __
Quantity: 50 Preps
Target Entry: __
Cell Line: __
Druggable Target: __
Accession Number:
__
Cell State:
__
Cytoplasmic Tail:
__
For Use With (Application):
Genotyping, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing, PCR
Human Protein Class:
__
Product Line:
PureLink™
Readout:
__
Type:
__
System Type:
__
Assay Entry:
__
Content And Storage:
• 10 mL PureLink™ Genomic Lysis/Binding Buffer• 9 mL PureLink™ Genomic Digestion Buffer• 10 mL PureLink™ Genomic Wash Buffer 1• 7.5 mL PureLink™ Genomic Wash Buffer 2• 10 mL PureLink™ Genomic Elution Buffer• 1 mL RNase A (20 mg/mL)• 1 mL Proteinase K (20 mg/mL)• 50 each PureLink™ Spin Columns with Collection Tubes• 100 PureLink™ Collecion Tubes (2.0 mL)This kit contains enough reagents for 50 DNA preps. Store all components at room temperature. For long-term storage, Proteinase K and RNase A may be stored at 4°C.
Detection Method:
__
Gene Symbol:
__
Population Status:
__
Quantity:
50 Preps
Target Entry:
__
Cell Line:
__
Druggable Target:
__
Accession Number: __
Cell State: __
Cytoplasmic Tail: __
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Human Protein Class: __
Product Line: __
Readout: __
Type: __
System Type: __
Assay Entry: __
Content And Storage: • 50 mL PureLink Genomic Lysis/Binding Buffer; room temperature• 45 mL PureLink Genomic Digestion Buffer; room temperature• 50 mL PureLinkGenomic Wash Buffer 1; room temperature• 37.5 mL PureLink Genomic Wash Buffer 2; room temperature• 50 mL PureLink Genomic Elution Buffer; room temperature• 5 mL RNase A (20 mg/mL); room temperature• 5 mL Proteinase K (20 mg/mL); room temperature, or 4°C for long-term storage• 5 x 50 PureLink Spin Columns with Collection Tubes; room temperature• 5 x 100 PureLink Collection Tubes; room temperature
Detection Method: __
Gene Symbol: __
Population Status: __
Quantity: 250 Preps
Target Entry: __
Cell Line: __
Druggable Target: __
Accession Number:
__
Cell State:
__
Cytoplasmic Tail:
__
For Use With (Application):
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Human Protein Class:
__
Product Line:
__
Readout:
__
Type:
__
System Type:
__
Assay Entry:
__
Content And Storage:
• 50 mL PureLink Genomic Lysis/Binding Buffer; room temperature• 45 mL PureLink Genomic Digestion Buffer; room temperature• 50 mL PureLinkGenomic Wash Buffer 1; room temperature• 37.5 mL PureLink Genomic Wash Buffer 2; room temperature• 50 mL PureLink Genomic Elution Buffer; room temperature• 5 mL RNase A (20 mg/mL); room temperature• 5 mL Proteinase K (20 mg/mL); room temperature, or 4°C for long-term storage• 5 x 50 PureLink Spin Columns with Collection Tubes; room temperature• 5 x 100 PureLink Collection Tubes; room temperature
Detection Method:
__
Gene Symbol:
__
Population Status:
__
Quantity:
250 Preps
Target Entry:
__
Cell Line:
__
Druggable Target:
__