K1830
Thermo Scientific™ Tango™ F2RL1-blaU2OS Cells
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1830-Each-of-1 | In Stock | ₹ 9,05,224.80 |
K1830 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,05,224.80
GST (18%): ₹ 1,62,940.464
Total Price: ₹ 10,68,165.264
Accession Number
NM_005242.3
Cell State
Dividing Cells
Cytoplasmic Tail
Tail from AVPR2 GPCR
For Use With (Application)
FRET
Human Protein Class
G-Protein Coupled Receptor (GPCR)
Product Line
GeneBLAzer™, Tango™
Readout
End Point
Cell Line
U2OS
Druggable Target
GPCR
Assay Entry
Cell-based beta-arrestin recruitment
Content And Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ F2RL1-bla U2OS cells• Tango™ F2RL1-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ F2RL1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method
Fluorescence
Gene Symbol
F2RL1
Population Status
Single Cell Clone Origin
Quantity
8 x 106Cells
Target Entry
F2RL1
System Type
Tango™
Related Products
Description
- The Tango™ F2RL1-bla U2OS cells contain the human Coagulation factor II (thrombin) receptor-like 1 (F2RL1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line
- This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element
- The Tango™ F2RL1-bla U2OS cells are functionally validated for Z' and EC50 concentrations of SLIGRL
- In addition, Tango™ F2RL1-bla U2OS cells have been tested for assay performance under variable conditions
- These data are found in the Validation & Assay Performance Summary
- For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
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Accession Number: NM_005242.3
Cell State: Dividing Cells
Cytoplasmic Tail: Tail from AVPR2 GPCR
For Use With (Application): FRET
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Product Line: GeneBLAzer™, Tango™
Readout: End Point
Cell Line: U2OS
Druggable Target: GPCR
Assay Entry: Cell-based beta-arrestin recruitment
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ F2RL1-bla U2OS cells• Tango™ F2RL1-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ F2RL1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method: Fluorescence
Gene Symbol: F2RL1
Population Status: Single Cell Clone Origin
Quantity: 8 x 106Cells
Target Entry: F2RL1
System Type: Tango™
Accession Number:
NM_005242.3
Cell State:
Dividing Cells
Cytoplasmic Tail:
Tail from AVPR2 GPCR
For Use With (Application):
FRET
Human Protein Class:
G-Protein Coupled Receptor (GPCR)
Product Line:
GeneBLAzer™, Tango™
Readout:
End Point
Cell Line:
U2OS
Druggable Target:
GPCR
Assay Entry:
Cell-based beta-arrestin recruitment
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ F2RL1-bla U2OS cells• Tango™ F2RL1-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ F2RL1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method:
Fluorescence
Gene Symbol:
F2RL1
Population Status:
Single Cell Clone Origin
Quantity:
8 x 106Cells
Target Entry:
F2RL1
System Type:
Tango™
Accession Number: __
Cell State: __
Cytoplasmic Tail: __
For Use With (Application): __
Human Protein Class: __
Product Line: __
Readout: __
Cell Line: __
Druggable Target: __
Assay Entry: __
Content And Storage: __
Detection Method: __
Gene Symbol: __
Population Status: __
Quantity: __
Target Entry: __
System Type: __
Accession Number:
__
Cell State:
__
Cytoplasmic Tail:
__
For Use With (Application):
__
Human Protein Class:
__
Product Line:
__
Readout:
__
Cell Line:
__
Druggable Target:
__
Assay Entry:
__
Content And Storage:
__
Detection Method:
__
Gene Symbol:
__
Population Status:
__
Quantity:
__
Target Entry:
__
System Type:
__
Accession Number: __
Cell State: __
Cytoplasmic Tail: __
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Human Protein Class: __
Product Line: __
Readout: __
Cell Line: __
Druggable Target: __
Assay Entry: __
Content And Storage: • 12.5 mL Resuspension Buffer R2; room temperature• 5 mL Precipitation Buffer N2; room temperature• 7.5 mL Binding Buffer B4; room temperature• 25 mL Wash Buffer W4; room temperature• 10 mL Wash Buffer W5; room temperature• 10 mL Elution Buffer; room temperature• 0.75 mL 20% SDS; room temperature• 0.75 mL RNase A; room temperature• 50 PureLink Spin Columns with Collection Tubes; room temperature• 50 Collection Tubes; room temperature
Detection Method: __
Gene Symbol: __
Population Status: __
Quantity: 50 Preps
Target Entry: __
System Type: __
Accession Number:
__
Cell State:
__
Cytoplasmic Tail:
__
For Use With (Application):
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Human Protein Class:
__
Product Line:
__
Readout:
__
Cell Line:
__
Druggable Target:
__
Assay Entry:
__
Content And Storage:
• 12.5 mL Resuspension Buffer R2; room temperature• 5 mL Precipitation Buffer N2; room temperature• 7.5 mL Binding Buffer B4; room temperature• 25 mL Wash Buffer W4; room temperature• 10 mL Wash Buffer W5; room temperature• 10 mL Elution Buffer; room temperature• 0.75 mL 20% SDS; room temperature• 0.75 mL RNase A; room temperature• 50 PureLink Spin Columns with Collection Tubes; room temperature• 50 Collection Tubes; room temperature
Detection Method:
__
Gene Symbol:
__
Population Status:
__
Quantity:
50 Preps
Target Entry:
__
System Type:
__
Accession Number: __
Cell State: __
Cytoplasmic Tail: __
For Use With (Application): Double vacuum/gas manifold and adapters
Human Protein Class: __
Product Line: __
Readout: __
Cell Line: __
Druggable Target: __
Assay Entry: __
Content And Storage: __
Detection Method: __
Gene Symbol: __
Population Status: __
Quantity: __
Target Entry: __
System Type: __
Accession Number:
__
Cell State:
__
Cytoplasmic Tail:
__
For Use With (Application):
Double vacuum/gas manifold and adapters
Human Protein Class:
__
Product Line:
__
Readout:
__
Cell Line:
__
Druggable Target:
__
Assay Entry:
__
Content And Storage:
__
Detection Method:
__
Gene Symbol:
__
Population Status:
__
Quantity:
__
Target Entry:
__
System Type:
__