Invitrogen™ ActinRed™ 555 ReadyProbes™ Reagent (Rhodamine phalloidin)

Description

• Formulated as room temperature-stable solution and comes in convenient dropper bottle: tip and drip2 drops/mL to stain cells High-affinity staining of F-actin with superior specificity compared to antibody methods Outstanding fluorescence performance (Ex/Em: 540/565nm) Ready-to-use liquid formulation: no need to dilute, weigh, or pipet Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low nonspecific binding Phalloidin binds F-actin with high selectivity, while TRITC provides red-orange fluorescence of unparalleled brightness and photostability Demonstrating very little nonspecific staining, allows high-contrast discrimination of actin staining Visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations Fully compatible with other fluorescent stains used in fixed-cell analyses, including antibodies, fixed-cell dyes, fluorescent proteins, and Qdot probe Validated and robust reagent for studying cytoskeleton in the context of stem cell differentiation, cancer biology, as marker in RNAi library screens, or target in drug discovery May be added directly to fixed cells in full media or buffer solutions Optimization may be needed for some cell types, conditions, and applications: In such cases, simply add more or fewer drops until the optimal staining intensity is obtained Detected through standard TRITC (Rhodamine/DiI/Cy3/Alexa Fluor 546) filters Cell Analysis, Cell Structure, Cytoskeleton