Invitrogen™ ActinGreen™ 488 ReadyProbes™ Reagent (AlexaFluor™ 488 phalloidin)

Description

• Formulated as a room temperature-stable solution in dropper bottle Tip and drip 2 drops/mL to stain cells for optimally bright and distinct nuclear counter-staining and incubate 15 to 30 minutes in most cases High-affinity staining of F-actin with superior specificity compared to antibody methods Outstanding fluorescence performance (Ex/Em: 495/518nm) No need to dilute, weigh, or pipet Phalloidin is bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding Phalloidin binds F-actin with high selectivity, while Alexa Fluor 488 dye provides green fluorescence of unparalleled brightness and photostability Can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations It is fully compatible with other fluorescent stains used in fixed-cell analyses, including antibodies, fixed-cell dyes, fluorescent proteins, and Qdot probes Role of cytoskeleton in cell division and cell motility makes it central not only to normal, as well as malignant processes (unchecked proliferation and invasiveness); cytoskeletal components are targets of several cancer and anti-inflammatory drugs: validated and robust reagent for related studies May be added directly to fixed cells in full media or buffer solutions Detected through standard GFP and FITC filter Cell Analysis, Cell Structure, Cytoskeleton