Invitrogen™ Image-iT™ Fixation/Permeabilization Kit

Description

• The Image-iT Fixation/Permeabilization Kit is a convenient-to-use fixation/permeabilization kit that contains all the necessary reagents to prepare cells for antibody staining and imaging
• The high-quality components help preserve cell morphology and reduce background staining and are ideal for use with antibody-based detection methods, including immunocytochemistry (ICC), immunofluorescence (IF), and immunohistochemistry (IHC)
• An easy-to-follow protocol is also provided
• Note: the 4% formaldehyde, methanol-free fixative solution included in this kit is also available for separate purchase (see Cat
• No
• R37814)
• Features of the Image-iT Fixation/Permeabilization Kit include: • A complete fixation/permeabilization kit with ready-to-use components and room temperature storage • Optimized for secondary antibody labeling, fixed-cell dye staining, and GFP applications • Reduced background staining The Image-iT Fixation/Permeabilization Kit contains: • Fixative: high purity 4% formaldehyde (methanol-free) in PBS, pH 7.3 • Permeabilization solution: 0.5% Triton X-100 • Blocking buffer: 3% BSA, fraction V, de-lipidated, New Zealand source, in DPBS • Wash solution: DPBS, no calcium, no magnesium