Invitrogen™ eBioscience™ Mouse Cytokine Positive Control Cells

Description

• Mouse splenocytes were stimulated with ConA (3μg/mL) for two days, followed by recombinant IL-2 (20ng/mL) and IL-4 (20ng/mL) for three days The cells were then restimulated with immobilized anti-mouse CD3 (10μg/mL) and soluble anti-mouse CD28 (2μg/mL) in the presence of Brefeldin A for 5 hours These cells contain subpopulations that express IL-2, IL-3, IL-4, IL-10, GM-CSF, and IFNγ The cells have been fixed with formaldehyde and are provided in a stabilizing buffer so that they may be stored at −80°C for up to six months or at 2 to 8°C for up to one month