Invitrogen™ NovaFluor™ CD4 Label Characterization Kits

Description

• NovaFluor CD4 Label Characterization kits are formulated to enable the testing of all available NovaFluors as direct conjugates of CD4
• These kits can be used to evaluate performance of NovaFluors on flow cytometers
• NovaFluor CD4 Label Characterization kits are formulated to enable the testing of available NovaFluor dyes as direct conjugates of CD4
• These kits can be used to characterize and evaluate the performance of NovaFluor dyes
• NovaFluor CD4 Label Characterization Kits include: • NovaFluor antibody conjugates of anti-human CD4 (clone SK3) or anti-mouse CD4 (clone GK1.5), 10 tests of each antibody conjugate per vial
• • CellBlox Blocking Buffer • Kit 1 dyes: NovaFluor Blue 530, NovaFluor Blue 610-30S, NovaFluor Blue 660-40S, NovaFluor Yellow 570, NovaFluor Yellow 610, NovaFluor Yellow 660 • Kit 2 dyes: NovaFluor Blue 510, NovaFluor Blue 555, NovaFluor Blue 610-70S, NovaFluor Blue 660-120S, NovaFluor Yellow 690, NovaFluor Yellow 700, NovaFluor Red 660, NovaFluor Red 685, NovaFluor Red 700 NovaFluor Dyes include the following features: • Unique spectral signatures designed with narrow emission and minimal cross-laser excitation • Decreased spillover/spread for higher biological resolution • Easily added to new and existing panels to fill in spectral gaps • Ideal for both conventional and spectral flow cytometers NovaFluor dyes are not compatible with DNA -binding dyes
• Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD), or DAPI
• Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes
• Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer
• Use this buffer whenever you are staining with NovaFluor conjugates, including single-color controls using cells
• Use 5 μL of CellBlox Blocking Buffer per stained cell sample containing 10 3 to 10 8 cells with 100 μL as a final staining volume.