Invitrogen™ Dynabeads™ Streptavidin for Target Enrichment

Description

• Dynabeads Streptavidin for Target Enrichment beads are 1-μm monosized, monodispersed, superparamagnetic beads covalently coupled to streptavidin and intended for enrichment of target sequences for library preparations for next-generation sequencing (NGS) workflows
• Benefits of Dynabeads Streptavidin for Target Enrichment beads include: • Performance —high sensitivity and reproducibility of results • Efficiency —fast binding kinetics and high binding capacity • Accuracy —excellent coverage, which enables high confidence in variant detection • Automation ready —easy automation for high-throughput needs on the KingFisher Purification System Consistency and reproducibility The monolayer of streptavidin leaves most of the biotin-binding sites available for the binding of free biotin and biotinylated ligands
• The specific and defined surface allows for efficient capture, separation, and downstream handling
• In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer help ensure negligible leakage, batch consistency, and reproducibility of results
• High binding capacity The size of the molecule and the biotinylation procedure will affect the binding capacity
• The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules
• With two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization, one mg of Dynabeads Streptavidin for Target Enrichment typically binds: • 950–1,500 pmol free biotin • ∼20 μg biotinylated ds-DNA • ∼400 pmol biotinylated ss-oligonucleotides Applications Thermo Fisher streptavidin-coupled Dynabeads magnetic beads have been used and cited for a wide variety of applications
• Although the beads can be used to isolate any biotinylated targets, these hydrophobic beads are particularly well suited for the enrichment of target sequences for library preparations for NGS workflows
• Dynabeads Streptavidin for Target Enrichment is a high-quality product that enables robust and reproducible NGS analysis results on any NGS platform
• Dynabeads Streptavidin for Target Enrichment efficiently captures DNA sequences of interest through specific hybridization via immobilization of target-specific biotinylated probes to the streptavidin on the bead surface
• The subsequent washing steps utilize magnetic separation to obtain high purity
• The starting sample can be nucleic acid extracted from chromatin immunoprecipitated samples (ChIP) or isolated pure nucleic acid
• Automation ready with the KingFisher Purification System The 1-μm magnetic beads are ideal for high-throughput enrichment
• This process can be automated using any of our KingFisher Sample Purification Systems or liquid handlers
• Commercial supply Our manufacturing sites are ISO 13485-certified
• Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply
• If you are in the process of customizing Dynabeads Streptavidin for Target Enrichment beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or see our Dynabeads OEM page.