Invitrogen™ Dynabeads™ Streptavidin for In Vitro Transcription

Description

• Dynabeads Streptavidin for In Vitro Transcription offers an innovative technology for simple, flexible, and automation-ready mRNA synthesis in scales ranging from microgram to gram
• The 1-μm monosized, monodispersed, paramagnetic beads are covalently coupled to streptavidin molecules, ready for binding to a biotinylated DNA template
• The workflow is designed to minimize the effort and cost of plasmid preparation while enabling storage and reuse of bead-template complex in a successive solid-phase in vitro transcription (IVT) reaction
• Benefits of Dynabeads Streptavidin for In Vitro Transcription beads include: Flexibility —choose a DNA template for IVT mRNA with greater flexibility of design and amplification Efficiency —reuse the DNA template (>6x) to reduce cost and minimize plasmid prep hands-on time, and reduce the risk of transfer of bacterial host DNA Scalability —swap from manual to automated workflow to match the required scale and throughput Quality —helps ensure a safe and flexible supply of magnetic beads to navigate from RNA-based drug discovery to commercial manufacturing without incurring extra time or costs from switching technology or raw materials Reusable bead-based IVT The biotinylated template is made by PCR amplification of the target sequence in a plasmid or a synthetic DNA construct
• This uses a biotinylated forward primer, located 30-100 base pairs upstream of the T7-promoter, and a non-biotinylated reverse primer
• As an alternative to PCR amplification, a linearized plasmid can be biotinylated by a fill-in reaction of biotin-dUTP in the 5' overhang sequence
• The biotinylated template is then immobilized directly to Dynabeads Streptavidin for IVT without the need for prior purification
• The bead-bound template is used directly in the IVT, then removed from the synthesized mRNA by magnetic separation
• The template can then be reused at least six times in consecutive IVT reactions
• The protocol is scalable from micrograms to grams of mRNA
• Automation ready with the KingFisher Purification System The 1-μm Dynabeads magnetic beads are ideal for high-throughput enrichment
• This process can be automated using any of the KingFisher Sample Purification Systems or liquid handlers
• Commercial supply Our manufacturing sites are ISO 13485-certified
• Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply
• If you are in the process of customizing Dynabeads Streptavidin for In Vitro Transcription beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or read more on our Dynabeads OEM page
• To learn about other solutions and support documentation available for GMP-compliant manufacturing of mRNA vaccines and therapeutics visit thermofisher.com/dynabeadsmrna.