Molecular Probes™ FluoSpheres™ Polystyrene Microspheres, 1.0 μm, orange fluorescent (540/560), for tracer studies

Description

• Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene
• Our Molecular Probes™ FluoSpheres™ beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy
• For tracking particles and cells, tracing fluid dynamics, discriminating the size dependence of uptake or transport in vivo , etc., we offer more concentrated, 0.04 μm diameter FluoSpheres™ microspheres preparations (5% solids) that lack the sodium azide preservative present in the majority of our FluoSpheres™ products
• The 0.04 μm particles are small enough to be microinjected or to be taken up by phagocytosis
• We also offer 1.0μm FluoSpheres™ microsphere preparations that contain a much higher dye content than our other FluoSpheres™ products
• This allows stronger signals to be generated using fewer microspheres per tracing experiment
• In many biological systems, the concentrated fluorescence and spherical shape of the FluoSpheres™ beads permit them to be detected against a relatively high but diffuse background fluorescence
• Typically for these types of studies, the microspheres and the fluorescent dyes they contain are first extracted from the tissue sample, and then the fluorescence is quantified on a spectrofluorometer or fluorescence microplate reader
• The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres
• FluoSpheres™ Microsphere Specifications: • Label (Ex/Em): Orange fluorescent (540/560) • Nominal bead diameter: 1.0 μm • Coupling surface: Carboxylate • Solids: 2% Characteristics of the Carboxylate Coupling Surface Carboxylate-modified FluoSpheres™ beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Choices for FluoSpheres™ Fluorescent Microspheres In addition to microspheres for tracing, investigate our complete offering of fluorescent microspheres products
• Among those products you’ll find beads with these variations: • Ten fluorescent colors • Ten nominal bead diameters: 0.02 μm, 0.04 μm, 0.1 μm, 0.2 μm, 0.5 μm, 1.0 μm, 2.0 μm, 4.0 μm, 10.0 μm, and 15.0 μm • Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine • Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum Choices for Unstained Microspheres We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications
• We’ll Make a Custom Microsphere Product for You We will prepare custom orders upon request
• For example, FluoSpheres™ beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications
• Our custom conjugation service is efficient and confidential, and we stand by quality of our work
• We are ISO 13485:2000 certified
• For Research Use Only
• Not intended for animal or human therapeutic or diagnostic use.