TrueGel3D Hydrogel Kits

Description

• General description: The gel is formed by stable thioether bonds between thiol groups on CD cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer, SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-DEXTRAN. SLO-Dextran does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step.The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.
• Application: TRUE7-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of mechanical stimuli on cell physiology and behavior. The SLO-DEXTRAN based hydrogels are used in microchannels and also in syringes for injection. These hydrogels are also employed to study cell migration of neutrophils, dendritic cells and lymphocytes.
• Features and Benefits: Slow gelling (takes 2.5 minutes to 6 minutes, depends on gel stiffness)Flexibility in modulating gel stiffnessContains MMP-cleavable crosslinkerAllow cell recovery and post-culture analyses Cell inert hydrogel (if not customized with RGD peptide)
• Components: TRUE7-1KT contains SLO-DEXTRAN solution in phosphate buffer and lyophilized CD cell-degradable crosslinker with reconstitution buffer and water.
• Caution: Avoid prolonged exposure of CD cell-degradable crosslinker to air.