Cystatin C human

Description

• General description: HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells. Cystatin C belongs to the cystatin superfamily.[1] Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by the elimination of small basic peptides or amino acids from the N-terminal end of the protein. This protein is coded by a housekeeping gene, CST3 located on human chromosome 20p11.21 and is generated by nucleated cells.[2]
• Application: Cystatin C human has been used as a standard in western blot assay.[3] It has also been used to study its effect on the endopeptidase activity of activated Trichobilharzia regenti isoform of cathepsin B1 peptidase (TrCB1).[4]
• Biochem/physiol Actions: Cystatin C is an inhibitor of cysteine proteases including cathepsin B which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR). Studies show its role in predicting new-onset or deteriorating cardiovascular disease.[5][6] It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease.[7] Cystatin C inhibits transforming growth factor β signaling in normal and cancer cells.[1]
• Physical form: Lyophilized from a 0.2 μm filtered solution of 1×PBS
• Analysis Note: The inhibitory function of cystatin c on papain′s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56 μg/mL to 50 μg/mL cystatin C in presence of 0.55 μM papain and 0.44 μM L-BAPA.
• Legal Information: HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc