Granulocyte-Macrophage Colony-Stimulating Factor human

Description

• General description: Granulocyte-Macrophage Colony-Stimulating Factor is mapped to human chromosome 5q21-q32.[1]
• Application: Granulocyte-Macrophage Colony-Stimulating Factor human has been used as a colony-stimulating cytokine in human acute myeloid leukemia cell line (OCI-M2) to test its effect on 5-azacitidin (AZA)-mediated DNA demethylation of upstream regulatory element (URE).[2]
• Biochem/physiol Actions: Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is an immune modulator and proinflammatory cytokine. It binds to the heteromeric cell-surface receptors present in the immune cells like granulocytes, endothelial cells and macrophages.[3] GM-CSF is implicated in inflammation associated disorder of the lung and renal system.[3]
• Caution: Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.
• Physical form: Lyophilized from a 0.2 μm filtered solution of 1x PBS.
• Preparation Note: HumanKine GM-CSF is expressed as a 15-36 kDa glycosylated monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.
• Analysis Note: The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
• Legal Information: HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc