EGF human

Description

• General description: The gene EGF (epidermal growth factor) is mapped to human chromosome 4q25-27.[1] The EGF gene encodes for a large type I membrane–anchored precursor protein which is cleaved into pro-EGF for generation of peptide hormone EGF. The mRNA is expressed in kidney, salivary gland, cerebrum and prostate.[2] Recombinant human EGF is a 6.2kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds.
• Application: EGF (epidermal growth factor) human has been used in the culture medium for the maintenance of MCF10A cells (human mammary epithelial cells).[2]
• Biochem/physiol Actions: EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells.[3] Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing.[4][5] EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.[6] This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). Mutation in the EGF gene might be associated with risk of non- small cell lung adenocarcinoma.[1] It is also involved in anaplastic thyroid cancer and renal hypomagnesemia. It controls cell differentiation and neurotrophic processes.[7][8]
• Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
• Physical form: Lyophilized with no additives.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.