IFN-γ human

Description

• General description: IFN-γ (interferon γ) is an acid-labile glycosylated interferon produced by CD4 and CD8 T lymphocytes as well as activated NK (natural killer) cells.[1][2][3] IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC (major histocompatibility complex) proteins. This promotes the presentation of antigen to T-helper (CD4+) cells.[4] The gene is mapped to human chromosome 12q15.[5] Human IFN-γ is species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.7kDa protein containing 143 amino acid residues.
• Application: IFN-γ (interferon γ)-human has been used for the generation of inflammatory M1 cells from human monocytic cell line, THP1.[6][7]
• Biochem/physiol Actions: IFN-γ (interferon γ) signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response.[8][9] It can suppress the generation of anti-inflammatory cytokines and enhance the secretion of proinflammatory cytokines.[10] Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK (natural killer) cells and neutrophils.[11][12] It is responsible for tumor rejection and is capable of killing tumor cells by autophagy or apoptosis.[3] IFN-γ is also involved in Hepatitis E virus disease pathogenesis.[13]
• Sequence: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
• Physical form: Lyophilized from 10 mM Sodium Phosphate, pH 7.4.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.