M-CSF from mouse

Description

• General description: M-CSF (macrophage colony-stimulating factor) is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts.[1] The protein works through its receptor CSF1R (colony stimulating factor 1 receptor).[2] The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant murine M-CSF is a 36.4kDa homodimeric protein containing two 156 amino acid polypeptide subunits.
• Biochem/physiol Actions: M-CSF (macrophage colony-stimulating factor) is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells.[1] M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility.[3] It is clinically used in the treatment of infection, malignancies and atherosclerosis.[4] It facilitates hematopoietic recovery after bone marrow transplantation. M-CSF also works as a pleiotropic growth factor and plays a crucial role in reproduction and organogenesis. Absence of it can result in reduced growth, neurological and reproductive problems as well as developmental defects in the mammary gland, bone and pancreas.[5]
• Sequence: MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP
• Physical form: 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.