PDGF-BB from mouse

Description

• General description: PDGFs (platelet-derived growth factors) are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains.[1] Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB.[2] Recombinant murine PDGF-BB is a 24.4kDa disulfide-linked homodimer of two B chains (218 total amino acids).
• Application: PDGF-BB from mouse has been used as a chemotactic factor on vascular smooth muscle cell.[3]
• Biochem/physiol Actions: The three naturally occurring PDGFs (platelet-derived growth factors); PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells.[1][4] The PDGFs are stored in platelet α-granules and are released upon platelet activation.[5] The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development.[6][7][8] Heterozygous mutation in PDGFB is associated with primary familial brain calcification.[9] It is also associated with angiogenesis for osteogenesis. PDGFB induces migration of endothelial progenitor cells and mesenchymal stem cells.[10]
• Sequence: MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT
• Physical form: Lyophilized from 10 mM Sodium Citrate, pH 4.0.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.