SRP3196

EGF from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Manufacturer: Sigma Aldrich

Synonym(S): Epidermal Growth Factor, URG, Urogastrone

Select a Size

Pack Size SKU Availability Price
500 μG SRP3196-500-μG In Stock ₹ 35,094.65

SRP3196 - 500 μG

₹ 35,094.65

In Stock

Quantity

1

Base Price: ₹ 35,094.65

GST (18%): ₹ 6,317.037

Total Price: ₹ 41,411.687

biological source

mouse

recombinant

expressed in E. coli

Assay

≥98% (HPLC)≥98% (SDS-PAGE)

form

lyophilized

potency

0.1 ng/mL

mol wt

6.0 kDa

packaging

pkg of 500 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

off-white to yellow

Description

  • General description: EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells.[1] Recombinant murine EGF is a 6kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide-bonds.
  • Application: EGF from mouse has also been used in the DMEM/F12 media for growing mouse kidney cells.[2]
  • Biochem/physiol Actions: EGF (epidermal growth factor) has been shown to inhibit gastric secretion, and to be involved in wound healing.[3][4] EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.[5] This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). EGF plays an important role in embryogenesis and cardiogenesis.[6][7]
  • Sequence: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
  • Physical form: Lyophilized from 10 mM Sodium Phosphate, pH 8.2.
  • Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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mouse

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expressed in E. coli

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≥98% (HPLC)≥98% (SDS-PAGE)

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lyophilized

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off-white to yellow

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