SRP3196
EGF from mouse
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Manufacturer: Sigma Aldrich
Synonym(S): Epidermal Growth Factor, URG, Urogastrone
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 μG | SRP3196-500-μG | In Stock | ₹ 35,094.65 |
SRP3196 - 500 μG
In Stock
Quantity
1
Base Price: ₹ 35,094.65
GST (18%): ₹ 6,317.037
Total Price: ₹ 41,411.687
biological source
mouse
recombinant
expressed in E. coli
Assay
≥98% (HPLC)≥98% (SDS-PAGE)
form
lyophilized
potency
0.1 ng/mL
mol wt
6.0 kDa
packaging
pkg of 500 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
off-white to yellow
Related Products
Description
- General description: EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells.[1] Recombinant murine EGF is a 6kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide-bonds.
- Application: EGF from mouse has also been used in the DMEM/F12 media for growing mouse kidney cells.[2]
- Biochem/physiol Actions: EGF (epidermal growth factor) has been shown to inhibit gastric secretion, and to be involved in wound healing.[3][4] EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.[5] This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). EGF plays an important role in embryogenesis and cardiogenesis.[6][7]
- Sequence: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
- Physical form: Lyophilized from 10 mM Sodium Phosphate, pH 8.2.
- Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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biological source: mouse
recombinant: expressed in E. coli
Assay: ≥98% (HPLC)≥98% (SDS-PAGE)
form: lyophilized
potency: 0.1 ng/mL
mol wt: 6.0 kDa
packaging: pkg of 500 μg
technique(s): cell culture | mammalian: suitable
impurities: <0.1 EU/μg endotoxin, tested
color: off-white to yellow
biological source:
mouse
recombinant:
expressed in E. coli
Assay:
≥98% (HPLC)≥98% (SDS-PAGE)
form:
lyophilized
potency:
0.1 ng/mL
mol wt:
6.0 kDa
packaging:
pkg of 500 μg
technique(s):
cell culture | mammalian: suitable
impurities:
<0.1 EU/μg endotoxin, tested
color:
off-white to yellow
biological source: mouse
recombinant: expressed in E. coli
Assay: ≥95% (HPLC)≥95% (SDS-PAGE)
form: lyophilized
potency: ≤0.5 ng/mL
mol wt: 15.9 kDa
packaging: pkg of 50 μg
technique(s): cell culture | mammalian: suitable
impurities: 0.0344 EU/μg endotoxin, tested
color: off-white to yellow
biological source:
mouse
recombinant:
expressed in E. coli
Assay:
≥95% (HPLC)≥95% (SDS-PAGE)
form:
lyophilized
potency:
≤0.5 ng/mL
mol wt:
15.9 kDa
packaging:
pkg of 50 μg
technique(s):
cell culture | mammalian: suitable
impurities:
0.0344 EU/μg endotoxin, tested
color:
off-white to yellow
biological source: mouse
recombinant: expressed in E. coli
Assay: ≥98% (HPLC)≥98% (SDS-PAGE)
form: lyophilized
potency: ≤0.2 ng/mL
mol wt: 15.1 kDa
packaging: pkg of 10 μg
technique(s): cell culture | mammalian: suitable
impurities: <0.1 EU/μg endotoxin, tested
color: white to off-white
biological source:
mouse
recombinant:
expressed in E. coli
Assay:
≥98% (HPLC)≥98% (SDS-PAGE)
form:
lyophilized
potency:
≤0.2 ng/mL
mol wt:
15.1 kDa
packaging:
pkg of 10 μg
technique(s):
cell culture | mammalian: suitable
impurities:
<0.1 EU/μg endotoxin, tested
color:
white to off-white
biological source: mouse
recombinant: expressed in E. coli
Assay: ≥98% (HPLC)≥98% (SDS-PAGE)
form: lyophilized
potency: ≤0.05 ng/mL
mol wt: 14.2 kDa
packaging: pkg of 20 μg
technique(s): cell culture | mammalian: suitable
impurities: <0.1 EU/μg endotoxin, tested
color: white to off-white
biological source:
mouse
recombinant:
expressed in E. coli
Assay:
≥98% (HPLC)≥98% (SDS-PAGE)
form:
lyophilized
potency:
≤0.05 ng/mL
mol wt:
14.2 kDa
packaging:
pkg of 20 μg
technique(s):
cell culture | mammalian: suitable
impurities:
<0.1 EU/μg endotoxin, tested
color:
white to off-white