SRP3200
GDNF from mouse
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Manufacturer: Sigma Aldrich
Synonym(S): ATF-1, Glial-Derived Neurotrophic Factor
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 μG | SRP3200-10-μG | In Stock | ₹ 34,943.10 |
SRP3200 - 10 μG
In Stock
Quantity
1
Base Price: ₹ 34,943.10
GST (18%): ₹ 6,289.758
Total Price: ₹ 41,232.858
biological source
mouse
recombinant
expressed in E. coli
Assay
≥98% (HPLC)≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.2 ng/mL
mol wt
15.1 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
Description
- General description: GDNF (glial cell line derived neurotrophic factor) is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin.[1] These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures.[2] The functional murine GDNF ligand is a disulfide-linked homodimer, of two 15.1kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
- Application: GDNF from mouse has been used for stimulating primary murine cultures of dorsal root ganglion (DRG) neurons.[3]
- Biochem/physiol Actions: GDNF (glial cell line derived neurotrophic factor) signals through a multicomponent receptor system, composed of a RET (receptor tyrosine kinase) and one of the four GFR-α (α1-α4) receptors (GDNF family receptors).[5] GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons.[6] Using Parkinson′s disease monkey model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.[7] It might also be involved in the treatment of non-alcoholic fatty liver disease.[8] GDNF is needed for proper spermatogonial stem cell survival and proliferation.[9]
- Sequence: MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI
- Physical form: Lyophilized from 10 mM Sodium Citrate.
- Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
- Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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biological source: mouse
recombinant: expressed in E. coli
Assay: ≥98% (HPLC)≥98% (SDS-PAGE)
form: lyophilized
potency: ≤0.2 ng/mL
mol wt: 15.1 kDa
packaging: pkg of 10 μg
technique(s): cell culture | mammalian: suitable
impurities: <0.1 EU/μg endotoxin, tested
color: white to off-white
biological source:
mouse
recombinant:
expressed in E. coli
Assay:
≥98% (HPLC)≥98% (SDS-PAGE)
form:
lyophilized
potency:
≤0.2 ng/mL
mol wt:
15.1 kDa
packaging:
pkg of 10 μg
technique(s):
cell culture | mammalian: suitable
impurities:
<0.1 EU/μg endotoxin, tested
color:
white to off-white
biological source: mouse
recombinant: expressed in E. coli
Assay: ≥98% (HPLC)≥98% (SDS-PAGE)
form: lyophilized
potency: ≤0.05 ng/mL
mol wt: 14.2 kDa
packaging: pkg of 20 μg
technique(s): cell culture | mammalian: suitable
impurities: <0.1 EU/μg endotoxin, tested
color: white to off-white
biological source:
mouse
recombinant:
expressed in E. coli
Assay:
≥98% (HPLC)≥98% (SDS-PAGE)
form:
lyophilized
potency:
≤0.05 ng/mL
mol wt:
14.2 kDa
packaging:
pkg of 20 μg
technique(s):
cell culture | mammalian: suitable
impurities:
<0.1 EU/μg endotoxin, tested
color:
white to off-white
biological source: mouse
recombinant: expressed in E. coli
Assay: ≥98% (HPLC)≥98% (SDS-PAGE)
form: lyophilized
potency: __
mol wt: 19.8 kDa
packaging: pkg of 20 μg
technique(s): cell culture | mammalian: suitable
impurities: <0.1 EU/μg endotoxin, tested
color: white to off-white
biological source:
mouse
recombinant:
expressed in E. coli
Assay:
≥98% (HPLC)≥98% (SDS-PAGE)
form:
lyophilized
potency:
__
mol wt:
19.8 kDa
packaging:
pkg of 20 μg
technique(s):
cell culture | mammalian: suitable
impurities:
<0.1 EU/μg endotoxin, tested
color:
white to off-white
biological source: mouse
recombinant: expressed in CHO cells
Assay: ≥98% (HPLC)≥98% (SDS-PAGE)
form: lyophilized
potency: ≤0.1 ng/mL ED50
mol wt: 40 kDa
packaging: pkg of 10 μg
technique(s): cell culture | mammalian: suitable
impurities: <0.1 EU/μg endotoxin, tested
color: __
biological source:
mouse
recombinant:
expressed in CHO cells
Assay:
≥98% (HPLC)≥98% (SDS-PAGE)
form:
lyophilized
potency:
≤0.1 ng/mL ED50
mol wt:
40 kDa
packaging:
pkg of 10 μg
technique(s):
cell culture | mammalian: suitable
impurities:
<0.1 EU/μg endotoxin, tested
color:
__