NT-3 human

Description

• General description: NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family.[1] This protein is composed of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues.[2]
• Biochem/physiol Actions: NT3 functions on two structurally unrelated receptor types- the p75 neurotrophin receptor (p75NTR) and the receptor tyrosine kinase (TRK). It promotes the metastatic invasion of brain by breast cancer cells, by promoting the EMT (epithelial-mesenchymal transition) transition of breast cancer cells and suppressing the immune response in brain.[1] This protein is responsible for the indction and survival of neurite outgrowth from a subset of neural crest (NC) and placode-derived neurons.[3]Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kDa polypeptide monomers (240 total amino acid residues).
• Physical form: Lyophilized with no additives.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.