M4642
Minimum Essential Medium Eagle
With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Manufacturer: Sigma Aldrich
Synonym(S): EMEM, MEM
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 L | M4642-10-L | In Stock | ₹ 4,070.20 |
| 50 L | M4642-50-L | In Stock | ₹ 17,753.00 |
M4642 - 10 L
In Stock
Quantity
1
Base Price: ₹ 4,070.20
GST (18%): ₹ 732.636
Total Price: ₹ 4,802.836
form
powder
technique(s)
cell culture | mammalian: suitable
components
glucose: yesHanks’ salts (2% CO2): yesL-glutamine: yesEarle’s salts (5% CO2): noNaHCO3: nophenol red: yessodium pyruvate: nostable glutamine: noHEPES: no
shipped in
ambient
storage temp.
2-8°C
Related Products
Description
- General description: Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has high concentrations of amino acids, so the medium closely approximates the protein concentration of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has increased the applicability of this medium. This medium lacks calcium that permits the growth of cells in suspension. Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.
- Application: Minimum Essential Medium Eagle (MEM) has been used:as a component of the Hank′s MEM (HMEM)-Pipes buffer to induce anaphylatoxin induced-ear swelling in mice[1]as a component of the slice culture medium (SCM) for the incubation of mouse hippocampal slice cultures[2]to culture Müller cells from guinea pigs[3]
- Quantity: Formulated to contain 10.6 grams of powder per liter of medium.
- Reconstitution: Supplement with 0.35 g/L sodium bicarbonate.
SAFETY INFORMATION
WGK
WGK 1
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form: powder
technique(s): cell culture | mammalian: suitable
components: glucose: yesHanks’ salts (2% CO2): yesL-glutamine: yesEarle’s salts (5% CO2): noNaHCO3: nophenol red: yessodium pyruvate: nostable glutamine: noHEPES: no
shipped in: ambient
storage temp.: 2-8°C
form:
powder
technique(s):
cell culture | mammalian: suitable
components:
glucose: yesHanks’ salts (2% CO2): yesL-glutamine: yesEarle’s salts (5% CO2): noNaHCO3: nophenol red: yessodium pyruvate: nostable glutamine: noHEPES: no
shipped in:
ambient
storage temp.:
2-8°C
form: liquid
technique(s): cell culture | mammalian: suitable
components: sodium pyruvate: nophenol red: yesEarle’s salts (5% CO2): yesHEPES: noNaHCO3: yesL-glutamine: yes
shipped in: ambient
storage temp.: 2-8°C
form:
liquid
technique(s):
cell culture | mammalian: suitable
components:
sodium pyruvate: nophenol red: yesEarle’s salts (5% CO2): yesHEPES: noNaHCO3: yesL-glutamine: yes
shipped in:
ambient
storage temp.:
2-8°C
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form: powder
technique(s): HPLC: suitable
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storage temp.: 2-8°C
form:
powder
technique(s):
HPLC: suitable
components:
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shipped in:
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storage temp.:
2-8°C