M8042

Minimum Essential Medium Eagle

Alpha Modification, with nucleosides and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

Manufacturer: Sigma Aldrich

Synonym(S): EMEM, MEM

Select a Size

Pack Size SKU Availability Price
500 ML M8042-500-ML In Stock ₹ 6,787.28
6 X 500 ML M8042-6-X-500-ML In Stock ₹ 33,211.10
24 X 500 ML M8042-24-X-500-ML In Stock ₹ 1,23,091.08

M8042 - 500 ML

₹ 6,787.28

In Stock

Quantity

1

Base Price: ₹ 6,787.28

GST (18%): ₹ 1,221.71

Total Price: ₹ 8,008.99

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

Hanks’ salts (2% CO2): noglucose: yessodium pyruvate: yesphenol red: yesHEPES: noL-glutamine: nostable glutamine: noEarle’s salts (5% CO2): yesNaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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Description

  • General description: Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.[1]This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Often used in cytogenetic analysis when supplemented with fetal bovine serum (FBS).
  • Application: Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell,[2] and osteoblast.[3]
  • Biochem/physiol Actions: MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.[6]
  • Reconstitution: Supplement with 0.292 g/L L-glutamine

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Hanks’ salts (2% CO2): noglucose: yessodium pyruvate: yesphenol red: yesHEPES: noL-glutamine: nostable glutamine: noEarle’s salts (5% CO2): yesNaHCO3: yes

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