ChromoCult® Listeria Agar Selective-Supplement

Description

• General description: ChromoCult® Listeria Agar Selective-Supplement contains a mixture of four antibiotics in lyophilized form. It largely inhibits the growth of accompanying bacteria by selective enrichment of Listeria spp. Amphotericin B reduces the growth of yeasts and molds. Polymyxin B inhibits the growth of Gram-negative bacteria. While nalidixic acid sodium salt mainly inhibits the growth of Gram-negative bacteria, it also inhibits some Gram-positive bacteria. Ceftazidime has a broad-spectrum activity against both Gram-negative and Gram-positive bacteria.
• Application: FRASER Listeria Selective Supplement is an additive for the preparation of ChromoCult® Listeria agar (base) for the selective growth of Listeria spp.
• Linkage: Replaces: 1.00432.0017
• Analysis Note: Growth promotion test according to ISO 11133.Inoculum on reference medium - Listeria monocytogenes ATCC 13932 (WDCM 00021): 10 - 100 - Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100 - Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100Recovery on test medium - Listeria monocytogenes ATCC 13932 (WDCM 00021): ≥ 70 % - Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 % - Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %Colonies - Listeria monocytogenes ATCC 13932 (WDCM 00021): blue-green; non-transparent zone - Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone - Listeria innocua ATCC 33090 (WDCM 00017): blue-greenGrowth - Escherichia coli ATCC 25922 (WDCM 00013): no growth - Escherichia coli ATCC 8739 (WDCM 00012): no growth - Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth - Enterococcus faecalis ATCC 29212 (WDCM 00087): no growthIncubation: Listeria 24-48 hrs. 37°C aerobic; other strains at least 48 hrs.
• Legal Information: CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany