Trypsin Inhibitor

Description

• General description: Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges.[1] It belongs to serpins − serine protease inhibitors family.[2]
• Specificity: Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).
• Application: Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.[3]
• Biochem/physiol Actions: Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic.[1] It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.[2]
• Specifications: Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)Isoelectric point: 4.5
• Unit Definition: 1 U = 270 S & T-units or 90 USP-units
• Preparation Note: Working concentration: 10 to 100 μg/mlWorking solution: Recommended solvent is distilled water.Stock solution: 1 mg/mlStorage conditions (working solution): -15 to -25 °CThe enzyme is stable for 6 months, frozen in aliquots.
• Reconstitution: Sensitive to heat, alkaline pH, and protein-precipitating compounds.
• Storage and Stability: Store at 2 to 8 °C. (Store dry!)
• Other Notes: For life science research only. Not for use in diagnostic procedures.