Hybridoma Fusion and Cloning Supplement

Description

• General description: Medium supplement composed of albumin, insulin, transferrin, cytokines, a cholesterol source, other defined organic and inorganic compounds and fetal calf serum (final concentration: 0.5%).HFCS contains human proteins. The raw material from which the human proteins were isolated, has been tested for the presence of Hepatitis B surface Antigen (HBsAg) and HIV-1/2 antibodies and found to be negative (according to current quality control procedures).
• Application: Hybridoma Fusion and Cloning Supplement (HFCS) is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. HFCS replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.[1][2]
• Physical form: Solution (50x concentrated, pH 7.4); filtered through 0.2 μm pore size membrane
• Preparation Note: Working concentration: HFCS (50x) is diluted 1:50 (v/v) with basal medium. It is strongly suggested that you use RPMI 1640. The final medium should also contain L-glutamine and sodium bicarbonate.
• Analysis Note: Biological activity: Assayed for high cloning efficiency of a hybridoma cell line.
• Other Notes: For life science research only. Not for use in diagnostic procedures.