Description

• General description: Bovine serum albumin is a protein which has a key role in regulating blood colloidal osmotic pressure. A water-soluble protein composed of 583 amino acids,[1][2] BSA has a calculated molecular weight of 66,430 Daltons. Six α-helices form three homologous domains of BSA. Depending on pH, BSA undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.[3]This BSA product is specifically evaluated and tested for the following properties:Very low protease content / protease-freeVery low fatty acid content / fatty acid-freeProtease-free BSA is especially useful for applications like:Enzyme assaysProtein-based assaysProtease-sensitive techniques that include:a. Enzyme immunoassay (EIA)b. Nucleic acid hybridizationc. Radioimmunoassay (RIA)
• Application: Bovine Serum Albumin has been used:as a constituent of medium used for the differentiation of human pluripotent stem cells[4]in enzyme-linked immunosorbent assay (ELISA) and sandwich ELISA[5] to permeabilize fetal cortical cells[6]This A7030 BSA has specifically been used as a component of blocking buffer in a peptide-based ELISA assay for analysis of COVID-19 / SARS-CoV-2 samples (Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020)).
• Biochem/physiol Actions: Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
• Features and Benefits: Heat shock fractionatedProtease-freeFatty acid-freeEssentially globulin-free
• Preparation Note: Prepared using heat shock fractionation