Description

• General description: Bovine serum albumin (BSA) is 66 kDa protein consisting of three domains with two subdomains under each. It is α-helical globular and a non-glycosylated protein.[1]
• Application: Bovine Serum Albumin has been used:as a blocking agent in enzyme linked immunosorbent assay (ELISA)(154)as a component of Dulbecco PBS medium for preantral follicles culture(155)as a component of modified Tris-buffered medium (mTBM) for oocyte insemination(156)
• Biochem/physiol Actions: Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids.[1] It is a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.[2] The cysteine and tyrosine residues of BSA have high affinity for metal binding.[1]
• Features and Benefits: Heat shock fractionatedSuitable for radioimmunoassay (RIA)
• Caution: May yield an insulin blank of up to 0.1 μunit per mg in certain procedures.
• Preparation Note: Prepared using heat shock fractionation