Human Collagen Type V

Description

• General description: Collagen type V is fibril-forming collagen that is located in the lung, bone, and fetal membranes along with type I collagen.[1] It is minor collagen present in the extracellular matrix.[2]
• Application: Human Collagen Type V has been used to study the interaction of high endothelial venule protein (hevin) with human collagen V by fluorescence polarization (FP) assay and surface plasmon resonance(SPR).[3] It has also been used to incubate the 96 well plate to determine the protein levels in media and cell lysates of fibroblasts.[1]
• Biochem/physiol Actions: Collagen type V acts as a regulator of collagen fibrillogenesis in the cornea and skin dermis. It interacts with matrix collagens and structural proteins thereby contributing to the structural integrity of tissues. Lower levels of type V collagen lead to loss of corneal transparency and Ehler Danlos syndrome.[2]
• Physical form: Liquid, in 0.1M acetic acid, pH 3.0. No preservatives added.
• Preparation Note: Purified by serial salt precipitations of a pepsin extraction of human fetal membranes and chromatography on DEAE-cellulose.
• Storage and Stability: Maintain at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze.
• Other Notes: Molecular composition: α1(V)]2 α2(V), native triple helix. Purity and retention of the native helical structure were monitored by SDS-PAGE, ORD measurement, and by reaction with anti-collagen type-specific monoclonal antibodies. Product Source: Human placenta, negative for HBsAg and HIV antibodies.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.