Description

• General description: β-Estradiol or estradiol-17 β (E2) is secreted majorly by the large preovulatory follicles in the ovary. In addition, estradiol can be synthesized de novo from cholesterol have androgens synthesized from cholesterol. The aromatization of androgen by enzyme aromatase (P450arom) results in the β-estradiol production.[1]
• Application: β-Estradiol has been used:as a component of Dulbecco′s modified Eagle medium (DMEM) for culturing mammary tumor cells[2]as a oestrogenic compound for testing neuroprotective effects[3]as a component of medium199 for culturing oocytes[4]
• Biochem/physiol Actions: β-Estradiol (E2) is a major premenopausal hormone in women and is essential for spermiogenesis, sperm maturation and motility in men.[5] Cultured bone marrow stem cells (BMSCs) have ability to synthesize E2 and could be exploited for treating estrogen deficiency.[1] It mediates physiological functions through estrogen receptor (ER) α and ERβ.[6]
• Physical form: powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw
• Reconstitution: To prepare a 20 μg/ml stock solution, add 1 ml absolute ethanol to 1 mg β-estradiol; gently swirl to dissolve; add 49 ml sterile medium while mixing.