Human Fibronectin

Description

• General description: Fibronectin is a class of high-molecular-weight adhesive glycoprotein,[1] predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The FN gene, which is highly conserved, gives rise to a minimum of 20 isoforms through alternative splicing in humans. These isoforms include notable variants such as cellular FN and plasma FN.[2] Plasma FN is primarily synthesized in the liver, whereas cellular FN which represents only 5% of the circulating fibronectins[3] is locally produced by various cell types.[2]
• Application: Fibronectin human plasma has been used: in neural selection medium to coat tissue culture surface for neuronal differentiation[4] to coat culture vessels for characterization of iPSC and iPSC‑derived BMEC in 2D cell culture[5] to coat culture supports for endothelial cell culture[6]
• Biochem/physiol Actions: Fibronectin plays a major role in biological processes. It interacts with surface receptors and other major ECM proteins like collagen to mediate cellular signals.[2] Fibronectin is involved in a variety of cellular functions, including adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation.[3] This glycoprotein can bind to integrins, collagens, fibrins and glycosaminoglycans (GAGs).[1] FN plays a crucial role in promoting cell cycle progression and proliferation. This interaction influences cyclins and immediate early response genes, contributing to cellular processes related to cell division and growth. FN is also involved in cell survival and protection from apoptosis in various cell types, including epithelial and endothelial cells and leukocytes. FN serves as a critical factor in promoting cell survival and preventing programmed cell death in these cellular contexts.[7] Mutations in the FN gene are associated with FN glomerulopathy and spondylometaphyseal dysplasia.[2] Fibronectin plays a significant role in the wound healing process, and in extracellular matrix (ECM) assembly, hence is considered suitable for tissue engineering applications.[1]
• Caution: The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.
• Preparation Note: This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C. In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.
• Disclaimer: RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.