Fibroblast Growth Factor-4 human

Description

• General description: Fibroblast Growth Factor-4 (FGF4) is a 19 kDa protein, belonging to the FGF family of proteins.[1] Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface.[2] There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34 kDa and they possess a conserved sequence of 120 amino acids.[3] The FGF4 gene is mapped to human chromosome 11q13.3.[4]
• Application: Fibroblast Growth Factor-4 human has been used:To maintain trophectoderm stem cells.[5]In neurogenic differentiation.[6] In hepatocyte differentiation.[7]
• Biochem/physiol Actions: Fibroblast growth factor-4 (FGF-4) is a 19 kDa protein identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development.
• Physical form: Lyophilized from a 0.2 μm filtered buffer solution containing a carrier protein.
• Analysis Note: The biological activity is measured by the dose-dependent stimulation of thymidine uptake using BaF3 cells expressing FGF receptors.