Description

• General description: Research area: Cell signaling Leptin (Ob) is a non-glycosylated polypeptide hormone.[1] It is produced primarily in adipocytes and the placenta.[2] The structure of leptin closely resembles members of the long-chain helical cytokine family. It is composed of four antiparallel α-helices interconnected by two long crossover links and one short loop. These structural elements are arranged in a left-handed helical bundle formation, resulting in a unique two-layer packing configuration.[1]
• Application: Leptin from mouse has been used:to check the effect of leptin on ovulation for treating infertility in obese mice[3]to check the effect of leptin on MGARP (mitochondria-localized glutamic acid-rich protein) expression in testis and follicular granular cells of mice[4]to study the morphometrical changes in mouse testicular tissue upon long term exposure to leptin[5]
• Biochem/physiol Actions: Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.Human and mouse leptin share ~84% sequence identity.
• Physical form: Lyophilized from a 0.2 μm filtered solution with 0.0045 mM NaHCO3
• Analysis Note: The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.