Description

• General description: Poly-D-Lysine Hydrobromide (PDL), a positively charged polymer consisting of lysine residues, where each lysine unit is associated with a hydrobromide molecule. This hydrobromide molecule gives the poly-D-lysine a unique characteristic, enabling it to form crystals and making it easily soluble in water-based solutions.
• Application: Poly d lysine hydrobromide coating has been used: In coating tissue culture plate wells and coverslips for immunofluorescence microscopy of primary neuronal culture to study neurological disorders associated with Zika virus infection.[1] To coat the coverslips for enhancing the attachment and proliferation of the myenteric cells isolated from the rat intestines.[2] In preparing surface of coverslips for cell attachment for studying the myenteric plexus of rat’s small intestine.[3]
• Biochem/physiol Actions: Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.
• Features and Benefits: Sterilized using γ-irradiation
• Components: Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
• Caution: Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.
• Preparation Note: This product has a molecular weight of 30,000-70,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.
• Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research