Description

• General description: Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties. Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.[1]
• Application: Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.[1]Poly-L-lysine hydrobromide has been used to adhere the dissociated cells of cortical tissues into the culture plates.[2] It has also been used to treat slides, to fix samples on it for immunohistochemical analysis.[3]
• Biochem/physiol Actions: Poly-L-lysine is employed as a coating substance to facilitate improved cell attachment to culture surfaces.[4] Research indicates that plates coated with poly-L-lysine (PLL) exhibit an accelerated cell growth rate.[5] When mesenchymal stem cells (MSCs) are cultured on PLL-coated substrates, it helps preserve their stemness properties and prevents senescence. PLL-coated plates promote enhanced MSC growth and up-regulation of genes associated with cell adhesion, differentiation, proliferation, and signaling. Furthermore, porous PLL-coated surfaces have been shown to enhance cell proliferation and support the osteogenic differentiation of MSCs.[6]
• Components: Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
• Caution: Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.
• Analysis Note: Molecular weight based on viscosity. Also assayed by MALLS.
• Other Notes: For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.