MCP-1/MCAF human

Description

• General description: Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF), also called the C-C motif chemokine ligand 2 (CCL2),[1] is an inflammatory cytokine.[2] The CCL2 gene is mapped to human chromosome 17q12.[1] CCL2 is present in epithelial, endothelial, mesangial and microglial cells.[3] Structurally, it dimerizes and has four regions of the ß-sheet.[3] Recombinant human MCP-1 is a 8.6 kDa protein containing 76 amino acid residues including the four highly conserved cysteine residues.[3] The MCP family cross-reacts across species.
• Application: Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF) has been used: as a chemokine supplement in the alpha minimal essential medium for culturing neurons to test its effect on JmjC histone demethylase (Jmjd2a) mRNA[2]as a component of Dulbecco′s Modified Eagle (DMEM) medium, as a positive chemoattractant control in HeLa cells for migration assay[4]as a chemoattractant to induced macrophage chemotaxis[5]
• Biochem/physiol Actions: Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF) acts as a human immunodeficiency virus 1 (HIV-1) suppressor.[3] The MCP-1 acts as a chemoattractant for T cells and natural killer (NK) cells.[1] It also plays a key role in the migration and infiltration of macrophages.[3] CCL2 shows under expression in high-grade ovarian serous carcinomas (HGOSCs) and is expressed less in epithelial ovarian cancer (EOC).[1]
• Sequence: QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT
• Physical form: Lyophilized with no additives.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.