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SRP3114

Midkine human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Manufacturer: Sigma Aldrich

Synonym(S): MK, NEGF-2

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Pack Size SKU Availability Price
20 μG SRP3114-20-μG In Stock ₹ 34,943.10

SRP3114 - 20 μG

₹ 34,943.10

In Stock

Quantity

1

Base Price: ₹ 34,943.10

GST (18%): ₹ 6,289.758

Total Price: ₹ 41,232.858

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)≥98% (SDS-PAGE)

form

lyophilized

mol wt

13.4 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

P21741

Description

  • General description: Recombinant human Midkine is a 13.4 kDa protein containing 123 amino acid residues including five intra-molecular disulfide bonds.
  • Application: Midkine human has been used in cell viability assay and cell proliferation assay to determine its association with EMT (epithelial-mesenchymal transition) and gemcitabine resistance in biliary tract cancer.[1]
  • Biochem/physiol Actions: Midkine (MK) and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK).[2] MK (midkine) plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development.[3] MK chemoattracts embryonic neurons, neutrophils and macrophages.[3][4] Signaling through the ALK (anaplastic lymphoma kinase) receptor MK exerts angiogenic, growth and survival activities during tumorigenesis.[5] MK is expressed predominantly during midgestation in the brain and several other organs and is down-regulated at birth.[5]
  • Sequence: VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD
  • Physical form: Lyophilized from 0.4x PBS, pH 7.4.
  • Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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biological source:
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recombinant:
expressed in E. coli
Assay:
≥98% (HPLC)≥98% (SDS-PAGE)
form:
lyophilized
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packaging:
pkg of 20 μg
technique(s):
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impurities:
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P21741

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