Transferrin human

Description

• General description: Transferrin is encoded by the TF gene that is mapped to human chromosome 3q22.1.[1] It is a glycoprotein containing homologous iron-binding domains at N-terminal and C-terminal ends.[2][3] Transferrin has a molecular weight of 80,000 Da and belongs to the transferrin superfamily, the members of which include lactoferrin, melanotransferrin, and ovotransferrin.[4]
• Application: To supplement neurosphere culture medium and sphere differentiation medium.[5]
• Biochem/physiol Actions: Transferrin is an iron-binding glycoprotein that primarily functions in the transport of plasma iron in vertebrates. It may also have a secondary function of facilitating cell proliferation. The binding of ferric iron to transferrin requires the presence of an anion, that functions as a bridge between the metal and the protein.[6][7][8]
• Physical form: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.