Description

• Amino Acid Sequence: Val-Val-Ser-His-Phe-Asn-Asp-Cys-Pro-Asp-Ser-His-Thr-Gln-Phe-Cys-Phe-His-Gly-Thr-Cys-Arg-Phe-Leu-Val-Gln-Glu-Asp-Lys-Pro-Ala-Cys-Val-Cys-His-Ser-Gly-Tyr-Val-Gly-Ala-Arg-Cys-Glu-His-Ala-Asp-Leu-Leu-Ala [Disulfide bridges: 8-21; 16-32; 34-43]
• General description: The TNFα (tumor necrosis factor α) gene is mapped to human chromosome 6p21.33.[1] TNFα is a member of TNF superfamily.[2]
• Application: Transforming Growth Factor-α human has been used:to determine the ideal concentration of growth factors to be used in the culture medium that supports sheep preantral follicles[3]in human hepatocytes cell culture and keratinocyte cell culture[4][5]to induce morphogenesis in primary mouse organoids[2]
• Biochem/physiol Actions: The TNFA gene is significantly associated with the regulation of innate and adaptive immune responses.[6] Polymorphism of TNFA gene is known to cause acute motor axonal neuropathy.[7] The cytokine release and gene action is associated with epithelial cells, myeloid cells, endothelial, as well as tumor cells. Tumor cells are known to release TNFA, specifically in response to chemotherapy.[6]
• Physical form: Lyophilized from a 0.2 μm filtered buffered solution.
• Analysis Note: The biological activity is measured by its ability to stimulate 3H-thymidine incorpation into BALB/c 3T3 cells.