Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse

Description

• General description: Stromal derived factor-1 (SDF-1) is localized to adipocytes.[1]
• Application: Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay.[2] It has also been used to study the SDF-1 release from chitosan-based nanoparticles.[3]
• Biochem/physiol Actions: SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..
• Physical form: Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.
• Preparation Note: Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.
• Analysis Note: The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.