Canine Chondrocytes: CnC (Cryovial)

Description

• General description: Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.Canine Chondrocytes (CnC) are derived from normal canine articular cartilage where they produce and maintain the extracellular matrix of cartilage. Following monolayer culturing, CnC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes and allow them to sustain normal proteoglycans production and retention. Because of the prevalence of degenerative joint disease in geriatric dogs, CnC can be a useful tool for veterinary practice. Canine Chondrocytes (CnC) were utilized to evaluate anti-inflammatory and anti-oxidant activity of Glyco-Flex III (Yáñez, 2008) and trans-pterostilbene (Remsberg, 2008). They were also used in material studies aimed to develop optimal surfaces/scaffolds for engineered tissues, cellular arrays, biosensors and lab-on-chip devices (Wright, 2012; Shaik 2013a,b).
• Cell Line Origin: Cartilage
• Application: production and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regeneration
• Components: Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSO
• Preparation Note: 1st passage, >500,000 cells in Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSOCan be cultured at least 10 doublings
• Subculture Routine: Please refer to the CnC Culture Protocol.