Gibco™ MEM, autoclavable, no glutamine, powder

Description

• Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media
• MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes
• We offer a variety of Gibco MEM modifications for a range of cell culture applications
• Find the right formulation using the media selector tool
• This MEM is modified as follows: With: Phenol Red, Earle's salts Without: L-glutamine, HEPES, Sodium Bicarbonate The complete formulation is available
• Gibco MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME)
• Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification
• To allow autoclaving this medium, choline chloride is substituted with choline bitartrate, phenol red is reduced, and succinic acid is added to prevent precipitation at low pH
• This MEM formulation contains Earle’s salts for use in a CO 2 incubator
• This product is made with Earle’s salts
• MEM contains no proteins, lipids, or growth factors
• Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO 2 environment to maintain physiological pH
• This autoclavable form of Gibco™ cell culture medium requires pH adjustment to 4.1–4.2 before autoclaving, following by sodium bicarbonate and L-glutamine supplementation and final pH adjustment to 7.2–7.4 (see protocol for details)
• Order Info Shipping Condition: Room Temperature