Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain
Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population
PI binds to DNA by intercalating between the bases with little or no sequence preference
In aqueous solution, the dye has excitation/emission maxima of 493/636 nm
Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ∼30–40 nm to the red and the fluorescence emission maximum is shifted ∼15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm
PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry
Learn more about propidium iodide, and propidium iodide containing products
SAFETY INFORMATION
Recommended Storage : Contains 1 vial propidium iodide (100 mg).Store at room temperature and protect from light.